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Super-resolution architecture of mammalian centriole distal appendages reveals distinct blade and matrix functional components

Author

Listed:
  • T. Tony Yang

    (Academia Sinica)

  • Weng Man Chong

    (Academia Sinica)

  • Won-Jing Wang

    (National Yang Ming University)

  • Gregory Mazo

    (Memorial Sloan-Kettering Cancer Center)

  • Barbara Tanos

    (Institute of Cancer Research)

  • Zhengmin Chen

    (Academia Sinica)

  • Thi Minh Nguyet Tran

    (Academia Sinica
    Vietnam Academy of Science and Technology)

  • Yi-De Chen

    (Academia Sinica)

  • Rueyhung Roc Weng

    (Academia Sinica
    National Taiwan University Hospital)

  • Chia-En Huang

    (Academia Sinica
    TFBS Bioscience, Inc.)

  • Wann-Neng Jane

    (Academia Sinica)

  • Meng-Fu Bryan Tsou

    (Memorial Sloan-Kettering Cancer Center)

  • Jung-Chi Liao

    (Academia Sinica
    National Taiwan University)

Abstract

Distal appendages (DAPs) are nanoscale, pinwheel-like structures protruding from the distal end of the centriole that mediate membrane docking during ciliogenesis, marking the cilia base around the ciliary gate. Here we determine a super-resolved multiplex of 16 centriole-distal-end components. Surprisingly, rather than pinwheels, intact DAPs exhibit a cone-shaped architecture with components filling the space between each pinwheel blade, a new structural element we term the distal appendage matrix (DAM). Specifically, CEP83, CEP89, SCLT1, and CEP164 form the backbone of pinwheel blades, with CEP83 confined at the root and CEP164 extending to the tip near the membrane-docking site. By contrast, FBF1 marks the distal end of the DAM near the ciliary membrane. Strikingly, unlike CEP164, which is essential for ciliogenesis, FBF1 is required for ciliary gating of transmembrane proteins, revealing DAPs as an essential component of the ciliary gate. Our findings redefine both the structure and function of DAPs.

Suggested Citation

  • T. Tony Yang & Weng Man Chong & Won-Jing Wang & Gregory Mazo & Barbara Tanos & Zhengmin Chen & Thi Minh Nguyet Tran & Yi-De Chen & Rueyhung Roc Weng & Chia-En Huang & Wann-Neng Jane & Meng-Fu Bryan Ts, 2018. "Super-resolution architecture of mammalian centriole distal appendages reveals distinct blade and matrix functional components," Nature Communications, Nature, vol. 9(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-04469-1
    DOI: 10.1038/s41467-018-04469-1
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    Cited by:

    1. Ting-Jui Ben Chang & Jimmy Ching-Cheng Hsu & T. Tony Yang, 2023. "Single-molecule localization microscopy reveals the ultrastructural constitution of distal appendages in expanded mammalian centrioles," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

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