IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v9y2018i1d10.1038_s41467-018-04441-z.html
   My bibliography  Save this article

A revised road map for the commitment of human cord blood CD34-negative hematopoietic stem cells

Author

Listed:
  • Keisuke Sumide

    (Kansai Medical University)

  • Yoshikazu Matsuoka

    (Kansai Medical University)

  • Hiroshi Kawamura

    (Kansai Medical University
    Kansai Medical University)

  • Ryusuke Nakatsuka

    (Kansai Medical University)

  • Tatsuya Fujioka

    (Kansai Medical University)

  • Hiroaki Asano

    (Kyoto Prefectural University of Medicine)

  • Yoshihiro Takihara

    (Hiroshima University
    Japanese Red Cross Osaka Blood Center)

  • Yoshiaki Sonoda

    (Kansai Medical University)

Abstract

We previously identified CD34-negative (CD34−) severe combined immunodeficiency (SCID)-repopulating cells as primitive hematopoietic stem cells (HSCs) in human cord blood. In this study, we develop a prospective ultra-high-resolution purification method by applying two positive markers, CD133 and GPI-80. Using this method, we succeed in purifying single long-term repopulating CD34− HSCs with self-renewing capability residing at the apex of the human HSC hierarchy from cord blood, as evidenced by a single-cell-initiated serial transplantation analysis. The gene expression profiles of individual CD34+ and CD34− HSCs and a global gene expression analysis demonstrate the unique molecular signature of CD34− HSCs. We find that the purified CD34− HSCs show a potent megakaryocyte/erythrocyte differentiation potential in vitro and in vivo. Megakaryocyte/erythrocyte progenitors may thus be generated directly via a bypass route from the CD34− HSCs. Based on these data, we propose a revised road map for the commitment of human CD34− HSCs in cord blood.

Suggested Citation

  • Keisuke Sumide & Yoshikazu Matsuoka & Hiroshi Kawamura & Ryusuke Nakatsuka & Tatsuya Fujioka & Hiroaki Asano & Yoshihiro Takihara & Yoshiaki Sonoda, 2018. "A revised road map for the commitment of human cord blood CD34-negative hematopoietic stem cells," Nature Communications, Nature, vol. 9(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-04441-z
    DOI: 10.1038/s41467-018-04441-z
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-018-04441-z
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-018-04441-z?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Kim Vanuytsel & Carlos Villacorta-Martin & Jonathan Lindstrom-Vautrin & Zhe Wang & Wilfredo F. Garcia-Beltran & Vladimir Vrbanac & Dylan Parsons & Evan C. Lam & Taylor M. Matte & Todd W. Dowrey & Sara, 2022. "Multi-modal profiling of human fetal liver hematopoietic stem cells reveals the molecular signature of engraftment," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-04441-z. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.