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Quantifying compressive forces between living cell layers and within tissues using elastic round microgels

Author

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  • Erfan Mohagheghian

    (Huazhong University of Science and Technology
    University of Illinois at Urbana-Champaign)

  • Junyu Luo

    (Huazhong University of Science and Technology)

  • Junjian Chen

    (Huazhong University of Science and Technology)

  • Gaurav Chaudhary

    (University of Illinois at Urbana-Champaign)

  • Junwei Chen

    (Huazhong University of Science and Technology
    University of Illinois at Urbana-Champaign)

  • Jian Sun

    (University of Illinois at Urbana-Champaign)

  • Randy H. Ewoldt

    (University of Illinois at Urbana-Champaign)

  • Ning Wang

    (Huazhong University of Science and Technology
    University of Illinois at Urbana-Champaign)

Abstract

Increasing evidence shows that mechanical stresses are critical in regulating cell functions, fate, and diseases. However, no methods exist that can quantify isotropic compressive stresses. Here we describe fluorescent nanoparticle-labeled, monodisperse elastic microspheres made of Arg-Gly-Asp-conjugated alginate hydrogels (elastic round microgels, ERMGs). We generate 3D displacements and calculate strains and tractions exerted on an ERMG. Average compressive tractions on an ERMG are 570 Pa within cell layers and 360 Pa in tumor-repopulating cell (TRC) colonies grown in 400-Pa matrices. 3D compressive tractions on a 1.4-kPa ERMG are applied by surrounding cells via endogenous actomyosin forces but not via mature focal adhesions. Compressive stresses are substantially heterogeneous on ERMGs within a uniform cell colony and do not increase with TRC colony sizes. Early-stage zebrafish embryos generate spatial and temporal differences in local normal and shear stresses. This ERMG method could be useful for quantifying stresses in vitro and in vivo.

Suggested Citation

  • Erfan Mohagheghian & Junyu Luo & Junjian Chen & Gaurav Chaudhary & Junwei Chen & Jian Sun & Randy H. Ewoldt & Ning Wang, 2018. "Quantifying compressive forces between living cell layers and within tissues using elastic round microgels," Nature Communications, Nature, vol. 9(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-04245-1
    DOI: 10.1038/s41467-018-04245-1
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    Cited by:

    1. Antoine Vian & Marie Pochitaloff & Shuo-Ting Yen & Sangwoo Kim & Jennifer Pollock & Yucen Liu & Ellen M. Sletten & Otger Campàs, 2023. "In situ quantification of osmotic pressure within living embryonic tissues," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    2. Johannes W Blumberg & Ulrich S Schwarz, 2022. "Comparison of direct and inverse methods for 2.5D traction force microscopy," PLOS ONE, Public Library of Science, vol. 17(1), pages 1-25, January.

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