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Single quantum dot tracking reveals the impact of nanoparticle surface on intracellular state

Author

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  • Mohammad U. Zahid

    (University of Illinois at Urbana-Champaign
    University of Illinois at Urbana-Champaign)

  • Liang Ma

    (University of Illinois at Urbana-Champaign
    University of Illinois at Urbana-Champaign)

  • Sung Jun Lim

    (University of Illinois at Urbana-Champaign
    University of Illinois at Urbana-Champaign
    DGIST)

  • Andrew M. Smith

    (University of Illinois at Urbana-Champaign
    University of Illinois at Urbana-Champaign
    University of Illinois at Urbana-Champaign)

Abstract

Inefficient delivery of macromolecules and nanoparticles to intracellular targets is a major bottleneck in drug delivery, genetic engineering, and molecular imaging. Here we apply live-cell single-quantum-dot imaging and tracking to analyze and classify nanoparticle states after intracellular delivery. By merging trajectory diffusion parameters with brightness measurements, multidimensional analysis reveals distinct and heterogeneous populations that are indistinguishable using single parameters alone. We derive new quantitative metrics of particle loading, cluster distribution, and vesicular release in single cells, and evaluate intracellular nanoparticles with diverse surfaces following osmotic delivery. Surface properties have a major impact on cell uptake, but little impact on the absolute cytoplasmic numbers. A key outcome is that stable zwitterionic surfaces yield uniform cytosolic behavior, ideal for imaging agents. We anticipate that this combination of quantum dots and single-particle tracking can be widely applied to design and optimize next-generation imaging probes, nanoparticle therapeutics, and biologics.

Suggested Citation

  • Mohammad U. Zahid & Liang Ma & Sung Jun Lim & Andrew M. Smith, 2018. "Single quantum dot tracking reveals the impact of nanoparticle surface on intracellular state," Nature Communications, Nature, vol. 9(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-04185-w
    DOI: 10.1038/s41467-018-04185-w
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    Cited by:

    1. Alessandra Stangherlin & Joseph L. Watson & David C. S. Wong & Silvia Barbiero & Aiwei Zeng & Estere Seinkmane & Sew Peak Chew & Andrew D. Beale & Edward A. Hayter & Alina Guna & Alison J. Inglis & Ma, 2021. "Compensatory ion transport buffers daily protein rhythms to regulate osmotic balance and cellular physiology," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    2. Benjamin Midtvedt & Jesús Pineda & Fredrik Skärberg & Erik Olsén & Harshith Bachimanchi & Emelie Wesén & Elin K. Esbjörner & Erik Selander & Fredrik Höök & Daniel Midtvedt & Giovanni Volpe, 2022. "Single-shot self-supervised object detection in microscopy," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

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