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High frequency neural spiking and auditory signaling by ultrafast red-shifted optogenetics

Author

Listed:
  • Thomas Mager

    (Max Planck Institute of Biophysics)

  • David Lopez de la Morena

    (University Medical Center Göttingen
    University of Göttingen)

  • Verena Senn

    (Max Planck Institute for Brain Research
    Ernst-Strüngmann-Institute for Neuroscience)

  • Johannes Schlotte

    (Max Planck Institute of Biophysics
    Biozentrum, University of Basel)

  • Anna D´Errico

    (Max Planck Institute of Biophysics
    Goethe Universität Frankfurt)

  • Katrin Feldbauer

    (Max Planck Institute of Biophysics
    Max-Planck-Institut für Herz- und Lungenforschung)

  • Christian Wrobel

    (University Medical Center Göttingen)

  • Sangyong Jung

    (University Medical Center Göttingen
    Biomedical Sciences Institutes, A*STAR)

  • Kai Bodensiek

    (University Medical Center Göttingen)

  • Vladan Rankovic

    (University Medical Center Göttingen
    Auditory Neuroscience and Optogenetics Group, German Primate Center)

  • Lorcan Browne

    (University Medical Center Göttingen
    Auditory Neuroscience and Optogenetics Group, German Primate Center
    University College London)

  • Antoine Huet

    (University Medical Center Göttingen
    Auditory Neuroscience and Optogenetics Group, German Primate Center)

  • Josephine Jüttner

    (Friedrich Miescher Institute for Biomedical Research)

  • Phillip G. Wood

    (Max Planck Institute of Biophysics)

  • Johannes J. Letzkus

    (Max Planck Institute for Brain Research)

  • Tobias Moser

    (University Medical Center Göttingen
    University of Göttingen
    Auditory Neuroscience and Optogenetics Group, German Primate Center)

  • Ernst Bamberg

    (Max Planck Institute of Biophysics)

Abstract

Optogenetics revolutionizes basic research in neuroscience and cell biology and bears potential for medical applications. We develop mutants leading to a unifying concept for the construction of various channelrhodopsins with fast closing kinetics. Due to different absorption maxima these channelrhodopsins allow fast neural photoactivation over the whole range of the visible spectrum. We focus our functional analysis on the fast-switching, red light-activated Chrimson variants, because red light has lower light scattering and marginal phototoxicity in tissues. We show paradigmatically for neurons of the cerebral cortex and the auditory nerve that the fast Chrimson mutants enable neural stimulation with firing frequencies of several hundred Hz. They drive spiking at high rates and temporal fidelity with low thresholds for stimulus intensity and duration. Optical cochlear implants restore auditory nerve activity in deaf mice. This demonstrates that the mutants facilitate neuroscience research and future medical applications such as hearing restoration.

Suggested Citation

  • Thomas Mager & David Lopez de la Morena & Verena Senn & Johannes Schlotte & Anna D´Errico & Katrin Feldbauer & Christian Wrobel & Sangyong Jung & Kai Bodensiek & Vladan Rankovic & Lorcan Browne & Anto, 2018. "High frequency neural spiking and auditory signaling by ultrafast red-shifted optogenetics," Nature Communications, Nature, vol. 9(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-04146-3
    DOI: 10.1038/s41467-018-04146-3
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    Cited by:

    1. Rodrigo G. Fernandez Lahore & Niccolò P. Pampaloni & Enrico Schiewer & M.-Marcel Heim & Linda Tillert & Johannes Vierock & Johannes Oppermann & Jakob Walther & Dietmar Schmitz & David Owald & Andrew J, 2022. "Calcium-permeable channelrhodopsins for the photocontrol of calcium signalling," Nature Communications, Nature, vol. 13(1), pages 1-18, December.

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