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An effector from the Huanglongbing-associated pathogen targets citrus proteases

Author

Listed:
  • Kelley Clark

    (University of California)

  • Jessica Yvette Franco

    (University of California)

  • Simon Schwizer

    (University of California
    University of California)

  • Zhiqian Pang

    (University of Florida)

  • Eva Hawara

    (University of California)

  • Thomas W. H. Liebrand

    (University of California)

  • Deborah Pagliaccia

    (University of California)

  • Liping Zeng

    (University of California
    University of California)

  • Fatta B. Gurung

    (Texas A&M University)

  • Pengcheng Wang

    (University of California)

  • Jinxia Shi

    (University of California
    Shanghai Normal University)

  • Yinsheng Wang

    (University of California)

  • Veronica Ancona

    (Texas A&M University)

  • Renier A. L. Hoorn

    (University of Oxford)

  • Nian Wang

    (University of Florida)

  • Gitta Coaker

    (University of California)

  • Wenbo Ma

    (University of California
    University of California)

Abstract

The citrus industry is facing an unprecedented challenge from Huanglongbing (HLB). All cultivars can be affected by the HLB-associated bacterium ‘Candidatus Liberibacter asiaticus’ (CLas) and there is no known resistance. Insight into HLB pathogenesis is urgently needed in order to develop effective management strategies. Here, we use Sec-delivered effector 1 (SDE1), which is conserved in all CLas isolates, as a molecular probe to understand CLas virulence. We show that SDE1 directly interacts with citrus papain-like cysteine proteases (PLCPs) and inhibits protease activity. PLCPs are defense-inducible and exhibit increased protein accumulation in CLas-infected trees, suggesting a role in citrus defense responses. We analyzed PLCP activity in field samples, revealing specific members that increase in abundance but remain unchanged in activity during infection. SDE1-expressing transgenic citrus also exhibit reduced PLCP activity. These data demonstrate that SDE1 inhibits citrus PLCPs, which are immune-related proteases that enhance defense responses in plants.

Suggested Citation

  • Kelley Clark & Jessica Yvette Franco & Simon Schwizer & Zhiqian Pang & Eva Hawara & Thomas W. H. Liebrand & Deborah Pagliaccia & Liping Zeng & Fatta B. Gurung & Pengcheng Wang & Jinxia Shi & Yinsheng , 2018. "An effector from the Huanglongbing-associated pathogen targets citrus proteases," Nature Communications, Nature, vol. 9(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-04140-9
    DOI: 10.1038/s41467-018-04140-9
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    Cited by:

    1. Hai-Jian Huang & Yi-Zhe Wang & Li-Li Li & Hai-Bin Lu & Jia-Bao Lu & Xin Wang & Zhuang-Xin Ye & Ze-Long Zhang & Yu-Juan He & Gang Lu & Ji-Chong Zhuo & Qian-Zhuo Mao & Zong-Tao Sun & Jian-Ping Chen & Ju, 2023. "Planthopper salivary sheath protein LsSP1 contributes to manipulation of rice plant defenses," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    2. Felix Homma & Jie Huang & Renier A. L. van der Hoorn, 2023. "AlphaFold-Multimer predicts cross-kingdom interactions at the plant-pathogen interface," Nature Communications, Nature, vol. 14(1), pages 1-13, December.

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