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Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene

Author

Listed:
  • Carolin Seuring

    (Deutsches Elektronen-Synchrotron
    The Hamburg Centre for Ultrafast Imaging)

  • Kartik Ayyer

    (Deutsches Elektronen-Synchrotron)

  • Eleftheria Filippaki

    (Deutsches Elektronen-Synchrotron)

  • Miriam Barthelmess

    (Deutsches Elektronen-Synchrotron)

  • Jean-Nicolas Longchamp

    (Physics Department of the University of Zurich)

  • Philippe Ringler

    (Biozentrum, University of Basel)

  • Tommaso Pardini

    (Lawrence Livermore National Laboratory)

  • David H. Wojtas

    (University of Canterbury)

  • Matthew A. Coleman

    (Lawrence Livermore National Laboratory)

  • Katerina Dörner

    (Deutsches Elektronen-Synchrotron)

  • Silje Fuglerud

    (Deutsches Elektronen-Synchrotron)

  • Greger Hammarin

    (Department of Chemistry and Molecular Biology)

  • Birgit Habenstein

    (CNRS, CBMN UMR5248, IECB, Université de Bordeaux)

  • Annette E. Langkilde

    (University of Copenhagen)

  • Antoine Loquet

    (CNRS, CBMN UMR5248, IECB, Université de Bordeaux)

  • Alke Meents

    (Deutsches Elektronen-Synchrotron)

  • Roland Riek

    (Laboratory of Physical Chemistry, ETH Zürich)

  • Henning Stahlberg

    (Biozentrum, University of Basel)

  • Sébastien Boutet

    (SLAC National Accelerator Laboratory)

  • Mark S. Hunter

    (SLAC National Accelerator Laboratory)

  • Jason Koglin

    (SLAC National Accelerator Laboratory)

  • Mengning Liang

    (SLAC National Accelerator Laboratory)

  • Helen M. Ginn

    (University of Oxford
    Harwell Science & Innovation Campus)

  • Rick P. Millane

    (University of Canterbury)

  • Matthias Frank

    (Lawrence Livermore National Laboratory)

  • Anton Barty

    (Deutsches Elektronen-Synchrotron)

  • Henry N. Chapman

    (Deutsches Elektronen-Synchrotron
    The Hamburg Centre for Ultrafast Imaging
    University of Hamburg)

Abstract

Here we present a new approach to diffraction imaging of amyloid fibrils, combining a free-standing graphene support and single nanofocused X-ray pulses of femtosecond duration from an X-ray free-electron laser. Due to the very low background scattering from the graphene support and mutual alignment of filaments, diffraction from tobacco mosaic virus (TMV) filaments and amyloid protofibrils is obtained to 2.7 Å and 2.4 Å resolution in single diffraction patterns, respectively. Some TMV diffraction patterns exhibit asymmetry that indicates the presence of a limited number of axial rotations in the XFEL focus. Signal-to-noise levels from individual diffraction patterns are enhanced using computational alignment and merging, giving patterns that are superior to those obtainable from synchrotron radiation sources. We anticipate that our approach will be a starting point for further investigations into unsolved structures of filaments and other weakly scattering objects.

Suggested Citation

  • Carolin Seuring & Kartik Ayyer & Eleftheria Filippaki & Miriam Barthelmess & Jean-Nicolas Longchamp & Philippe Ringler & Tommaso Pardini & David H. Wojtas & Matthew A. Coleman & Katerina Dörner & Silj, 2018. "Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene," Nature Communications, Nature, vol. 9(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-04116-9
    DOI: 10.1038/s41467-018-04116-9
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