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Aurora A-dependent CENP-A phosphorylation at inner centromeres protects bioriented chromosomes against cohesion fatigue

Author

Listed:
  • Grégory Eot-Houllier

    (Université Rennes 1)

  • Laura Magnaghi-Jaulin

    (Université Rennes 1)

  • Géraldine Fulcrand

    (Université Rennes 1)

  • François-Xavier Moyroud

    (Université Rennes 1)

  • Solange Monier

    (Université Rennes 1)

  • Christian Jaulin

    (Université Rennes 1)

Abstract

Sustained spindle tension applied to sister centromeres during mitosis eventually leads to uncoordinated loss of sister chromatid cohesion, a phenomenon known as “cohesion fatigue.” We report that Aurora A-dependent phosphorylation of serine 7 of the centromere histone variant CENP-A (p-CENP-AS7) protects bioriented chromosomes against cohesion fatigue. Expression of a non-phosphorylatable version of CENP-A (CENP-AS7A) weakens sister chromatid cohesion only when sister centromeres are under tension, providing the first evidence of a regulated mechanism involved in protection against passive cohesion loss. Consistent with this observation, p-CENP-AS7 is detected at the inner centromere where it forms a discrete domain. The depletion or inhibition of Aurora A phenocopies the expression of CENP-AS7A and we show that Aurora A is recruited to centromeres in a Bub1-dependent manner. We propose that Aurora A-dependent phosphorylation of CENP-A at the inner centromere protects chromosomes against tension-induced cohesion fatigue until the last kinetochore is attached to spindle microtubules.

Suggested Citation

  • Grégory Eot-Houllier & Laura Magnaghi-Jaulin & Géraldine Fulcrand & François-Xavier Moyroud & Solange Monier & Christian Jaulin, 2018. "Aurora A-dependent CENP-A phosphorylation at inner centromeres protects bioriented chromosomes against cohesion fatigue," Nature Communications, Nature, vol. 9(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-04089-9
    DOI: 10.1038/s41467-018-04089-9
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