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Small GTPases and BAR domain proteins regulate branched actin polymerisation for clathrin and dynamin-independent endocytosis

Author

Listed:
  • Mugdha Sathe

    (National Centre for Biological Science (TIFR))

  • Gayatri Muthukrishnan

    (National Centre for Biological Science (TIFR))

  • James Rae

    (University of Queensland
    University of Queensland)

  • Andrea Disanza

    (IFOM, Fondazione Istituto FIRC di Oncologia Molecolare
    University of Milan)

  • Mukund Thattai

    (National Centre for Biological Science (TIFR)
    National Centre for Biological Sciences (TIFR))

  • Giorgio Scita

    (IFOM, Fondazione Istituto FIRC di Oncologia Molecolare
    University of Milan)

  • Robert G. Parton

    (University of Queensland
    University of Queensland)

  • Satyajit Mayor

    (National Centre for Biological Science (TIFR)
    Institute for Stem Cell Biology and Regenerative Medicine)

Abstract

Using real-time TIRF microscopy imaging, we identify sites of clathrin and dynamin-independent CLIC/GEEC (CG) endocytic vesicle formation. This allows spatio-temporal localisation of known molecules affecting CG endocytosis; GBF1 (a GEF for ARF1), ARF1 and CDC42 which appear sequentially over 60 s, preceding scission. In an RNAi screen for BAR domain proteins affecting CG endocytosis, IRSp53 and PICK1, known interactors of CDC42 and ARF1, respectively, were selected. Removal of IRSp53, a negative curvature sensing protein, abolishes CG endocytosis. Furthermore, the identification of ARP2/3 complex at CG endocytic sites, maintained in an inactive state reveals a function for PICK1, an ARP2/3 inhibitor. The spatio-temporal sequence of the arrival and disappearance of the molecules suggest a mechanism for a clathrin and dynamin-independent endocytic process. Coincident with the loss of PICK1 by GBF1-activated ARF1, CDC42 recruitment leads to the activation of IRSp53 and the ARP2/3 complex, resulting in a burst of F-actin polymerisation potentially powering scission.

Suggested Citation

  • Mugdha Sathe & Gayatri Muthukrishnan & James Rae & Andrea Disanza & Mukund Thattai & Giorgio Scita & Robert G. Parton & Satyajit Mayor, 2018. "Small GTPases and BAR domain proteins regulate branched actin polymerisation for clathrin and dynamin-independent endocytosis," Nature Communications, Nature, vol. 9(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-03955-w
    DOI: 10.1038/s41467-018-03955-w
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    Cited by:

    1. Raluca Groza & Kita Valerie Schmidt & Paul Markus Müller & Paolo Ronchi & Claire Schlack-Leigers & Ursula Neu & Dmytro Puchkov & Rumiana Dimova & Claudia Matthaeus & Justin Taraska & Thomas R. Weikl &, 2024. "Adhesion energy controls lipid binding-mediated endocytosis," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

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