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PCGF5 is required for neural differentiation of embryonic stem cells

Author

Listed:
  • Mingze Yao

    (Guangzhou Medical University
    Chinese Academy of Sciences)

  • Xueke Zhou

    (Guangzhou Medical University
    Chinese Academy of Sciences)

  • Jiajian Zhou

    (the Chinese University of Hong Kong)

  • Shixin Gong

    (Guangzhou Medical University
    Chinese Academy of Sciences)

  • Gongcheng Hu

    (Guangzhou Medical University
    Chinese Academy of Sciences)

  • Jiao Li

    (Guangzhou Medical University
    Chinese Academy of Sciences)

  • Kaimeng Huang

    (Guangzhou Medical University
    Chinese Academy of Sciences)

  • Ping Lai

    (Guangzhou Medical University
    Chinese Academy of Sciences)

  • Guang Shi

    (Guangzhou Medical University
    Chinese Academy of Sciences)

  • Andrew P. Hutchins

    (Southern University of Science and Technology)

  • Hao Sun

    (the Chinese University of Hong Kong)

  • Huating Wang

    (the Chinese University of Hong Kong)

  • Hongjie Yao

    (Guangzhou Medical University
    Chinese Academy of Sciences)

Abstract

Polycomb repressive complex 1 (PRC1) is an important regulator of gene expression and development. PRC1 contains the E3 ligases RING1A/B, which monoubiquitinate lysine 119 at histone H2A (H2AK119ub1), and has been sub-classified into six major complexes based on the presence of a PCGF subunit. Here, we report that PCGF5, one of six PCGF paralogs, is an important requirement in the differentiation of mouse embryonic stem cells (mESCs) towards a neural cell fate. Although PCGF5 is not required for mESC self-renewal, its loss blocks mESC neural differentiation by activating the SMAD2/TGF-β signaling pathway. PCGF5 loss-of-function impairs the reduction of H2AK119ub1 and H3K27me3 around neural specific genes and keeps them repressed. Our results suggest that PCGF5 might function as both a repressor for SMAD2/TGF-β signaling pathway and a facilitator for neural differentiation. Together, our findings reveal a critical context-specific function for PCGF5 in directing PRC1 to control cell fate.

Suggested Citation

  • Mingze Yao & Xueke Zhou & Jiajian Zhou & Shixin Gong & Gongcheng Hu & Jiao Li & Kaimeng Huang & Ping Lai & Guang Shi & Andrew P. Hutchins & Hao Sun & Huating Wang & Hongjie Yao, 2018. "PCGF5 is required for neural differentiation of embryonic stem cells," Nature Communications, Nature, vol. 9(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-03781-0
    DOI: 10.1038/s41467-018-03781-0
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    Cited by:

    1. Yanjiang Liu & Gongcheng Hu & Shengxiong Yang & Mingze Yao & Zicong Liu & Chenghong Yan & Yulin Wen & Wangfang Ping & Juehan Wang & Yawei Song & Xiaotao Dong & Guangjin Pan & Hongjie Yao, 2023. "Functional dissection of PRC1 subunits RYBP and YAF2 during neural differentiation of embryonic stem cells," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    2. Xianchun Lan & Song Ding & Tianzhe Zhang & Ying Yi & Conghui Li & Wenwen Jin & Jian Chen & Kaiwei Liang & Hengbin Wang & Wei Jiang, 2022. "PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression," Nature Communications, Nature, vol. 13(1), pages 1-15, December.

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