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Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses

Author

Listed:
  • Gilberto A. Santiago

    (Centers for Disease Control and Prevention, National Centers for Emerging and Zoonotic Infectious Diseases)

  • Jesús Vázquez

    (Centers for Disease Control and Prevention, National Centers for Emerging and Zoonotic Infectious Diseases)

  • Sean Courtney

    (Centers for Disease Control and Prevention, National Centers for Emerging and Zoonotic Infectious Diseases)

  • Katia Y. Matías

    (Centers for Disease Control and Prevention, National Centers for Emerging and Zoonotic Infectious Diseases)

  • Lauren E. Andersen

    (Centers for Disease Control and Prevention, National Centers for Emerging and Zoonotic Infectious Diseases)

  • Candimar Colón

    (Centers for Disease Control and Prevention, National Centers for Emerging and Zoonotic Infectious Diseases)

  • Angela E. Butler

    (Centers for Disease Control and Prevention, National Centers for Emerging and Zoonotic Infectious Diseases)

  • Rebecca Roulo

    (Centers for Disease Control and Prevention, National Centers for Emerging and Zoonotic Infectious Diseases)

  • John Bowzard

    (Centers for Disease Control and Prevention, National Centers for Emerging and Zoonotic Infectious Diseases)

  • Julie M. Villanueva

    (Centers for Disease Control and Prevention, National Centers for Emerging and Zoonotic Infectious Diseases)

  • Jorge L. Muñoz-Jordan

    (Centers for Disease Control and Prevention, National Centers for Emerging and Zoonotic Infectious Diseases)

Abstract

The emergence and spread of Zika virus (ZIKV) presented a challenge to the diagnosis of ZIKV infections in areas with transmission of dengue (DENV) and chikungunya (CHIKV) viruses. To facilitate detection of ZIKV infections, and differentiate these infections from DENV and CHIKV, we developed the Trioplex real-time RT-PCR assay (Trioplex assay). Here, we describe the optimization of multiplex and singleplex formats of the assay for a variety of chemistries and instruments to facilitate global standardization and implementation. We evaluated the analytical performance of all Trioplex modalities for detection of these three pathogens in serum and whole blood, and for ZIKV in urine. The limit of detection for the three viruses and in different RNA-extraction modalities is near 103 genome copy equivalents per milliliter (GCE/mL). Simultaneous testing of more than one specimen type from each patient provides a 6.4% additional diagnostic sensitivity. Overall, the high sensitivity of the Trioplex assay demonstrates the utility of this assay ascertaining Zika cases.

Suggested Citation

  • Gilberto A. Santiago & Jesús Vázquez & Sean Courtney & Katia Y. Matías & Lauren E. Andersen & Candimar Colón & Angela E. Butler & Rebecca Roulo & John Bowzard & Julie M. Villanueva & Jorge L. Muñoz-Jo, 2018. "Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses," Nature Communications, Nature, vol. 9(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-03772-1
    DOI: 10.1038/s41467-018-03772-1
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    Cited by:

    1. Zhichen Xu & Dongjuan Chen & Tao Li & Jiayu Yan & Jiang Zhu & Ting He & Rui Hu & Ying Li & Yunhuang Yang & Maili Liu, 2022. "Microfluidic space coding for multiplexed nucleic acid detection via CRISPR-Cas12a and recombinase polymerase amplification," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

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