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Expansion of functional personalized cells with specific transgene combinations

Author

Listed:
  • Christoph Lipps

    (HZI – Helmholtz Centre for Infection Research
    Justus-Liebig University Giessen)

  • Franziska Klein

    (HZI – Helmholtz Centre for Infection Research)

  • Tom Wahlicht

    (HZI – Helmholtz Centre for Infection Research)

  • Virginia Seiffert

    (HZI – Helmholtz Centre for Infection Research)

  • Milada Butueva

    (HZI – Helmholtz Centre for Infection Research)

  • Jeannette Zauers

    (InSCREENeX GmbH)

  • Theresa Truschel

    (InSCREENeX GmbH)

  • Martin Luckner

    (InSCREENeX GmbH)

  • Mario Köster

    (HZI – Helmholtz Centre for Infection Research)

  • Roderick MacLeod

    (Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures)

  • Jörn Pezoldt

    (HZI – Helmholtz Centre for Infection Research)

  • Jochen Hühn

    (HZI – Helmholtz Centre for Infection Research)

  • Qinggong Yuan

    (Hannover Medical School
    Twincore - Centre for Experimental and Clinical Infection Research GmbH)

  • Peter Paul Müller

    (HZI – Helmholtz Centre for Infection Research)

  • Henning Kempf

    (Hannover Medical School, MHH)

  • Robert Zweigerdt

    (Hannover Medical School, MHH)

  • Oliver Dittrich-Breiholz

    (Medical School Hannover)

  • Thomas Pufe

    (RWTH Aachen University)

  • Rainer Beckmann

    (RWTH Aachen University)

  • Wolf Drescher

    (Aachen University Hospital, RWTH Aachen University
    Rummelsberg Hospital)

  • Jose Riancho

    (Hospital U.M. Valdecilla, University of Cantabria, IDIVAL)

  • Carolina Sañudo

    (Hospital U.M. Valdecilla, University of Cantabria, IDIVAL)

  • Thomas Korff

    (University Heidelberg)

  • Bertram Opalka

    (University Hospital Essen)

  • Vera Rebmann

    (University Hospital Essen)

  • Joachim R. Göthert

    (University Hospital Essen)

  • Paula M. Alves

    (Universidade Nova de Lisboa)

  • Michael Ott

    (Hannover Medical School
    Twincore - Centre for Experimental and Clinical Infection Research GmbH)

  • Roland Schucht

    (InSCREENeX GmbH)

  • Hansjörg Hauser

    (HZI – Helmholtz Centre for Infection Research)

  • Dagmar Wirth

    (HZI – Helmholtz Centre for Infection Research
    Hannover Medical School)

  • Tobias May

    (InSCREENeX GmbH)

Abstract

Fundamental research and drug development for personalized medicine necessitates cell cultures from defined genetic backgrounds. However, providing sufficient numbers of authentic cells from individuals poses a challenge. Here, we present a new strategy for rapid cell expansion that overcomes current limitations. Using a small gene library, we expanded primary cells from different tissues, donors, and species. Cell-type-specific regimens that allow the reproducible creation of cell lines were identified. In depth characterization of a series of endothelial and hepatocytic cell lines confirmed phenotypic stability and functionality. Applying this technology enables rapid, efficient, and reliable production of unlimited numbers of personalized cells. As such, these cell systems support mechanistic studies, epidemiological research, and tailored drug development.

Suggested Citation

  • Christoph Lipps & Franziska Klein & Tom Wahlicht & Virginia Seiffert & Milada Butueva & Jeannette Zauers & Theresa Truschel & Martin Luckner & Mario Köster & Roderick MacLeod & Jörn Pezoldt & Jochen H, 2018. "Expansion of functional personalized cells with specific transgene combinations," Nature Communications, Nature, vol. 9(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-03408-4
    DOI: 10.1038/s41467-018-03408-4
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