IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v9y2018i1d10.1038_s41467-018-03190-3.html
   My bibliography  Save this article

High-efficiency RNA-based reprogramming of human primary fibroblasts

Author

Listed:
  • Igor Kogut

    (University of Colorado School of Medicine
    University of Colorado School of Medicine)

  • Sandra M. McCarthy

    (University of Colorado School of Medicine
    University of Colorado School of Medicine)

  • Maryna Pavlova

    (University of Colorado School of Medicine
    University of Colorado School of Medicine)

  • David P. Astling

    (University of Colorado School of Medicine)

  • Xiaomi Chen

    (University of Colorado School of Medicine
    University of Colorado School of Medicine)

  • Ana Jakimenko

    (University of Colorado School of Medicine
    University of Colorado School of Medicine)

  • Kenneth L. Jones

    (University of Colorado School of Medicine)

  • Andrew Getahun

    (University of Colorado School of Medicine)

  • John C. Cambier

    (University of Colorado School of Medicine)

  • Anna M. G. Pasmooij

    (University Medical Center)

  • Marcel F. Jonkman

    (University Medical Center)

  • Dennis R. Roop

    (University of Colorado School of Medicine
    University of Colorado School of Medicine)

  • Ganna Bilousova

    (University of Colorado School of Medicine
    University of Colorado School of Medicine
    University of Colorado School of Medicine)

Abstract

Induced pluripotent stem cells (iPSCs) hold great promise for regenerative medicine; however, their potential clinical application is hampered by the low efficiency of somatic cell reprogramming. Here, we show that the synergistic activity of synthetic modified mRNAs encoding reprogramming factors and miRNA-367/302s delivered as mature miRNA mimics greatly enhances the reprogramming of human primary fibroblasts into iPSCs. This synergistic activity is dependent upon an optimal RNA transfection regimen and culturing conditions tailored specifically to human primary fibroblasts. As a result, we can now generate up to 4,019 iPSC colonies from only 500 starting human primary neonatal fibroblasts and reprogram up to 90.7% of individually plated cells, producing multiple sister colonies. This methodology consistently generates clinically relevant, integration-free iPSCs from a variety of human patient’s fibroblasts under feeder-free conditions and can be applicable for the clinical translation of iPSCs and studying the biology of reprogramming.

Suggested Citation

  • Igor Kogut & Sandra M. McCarthy & Maryna Pavlova & David P. Astling & Xiaomi Chen & Ana Jakimenko & Kenneth L. Jones & Andrew Getahun & John C. Cambier & Anna M. G. Pasmooij & Marcel F. Jonkman & Denn, 2018. "High-efficiency RNA-based reprogramming of human primary fibroblasts," Nature Communications, Nature, vol. 9(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-03190-3
    DOI: 10.1038/s41467-018-03190-3
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-018-03190-3
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-018-03190-3?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Claire Vinel & Gabriel Rosser & Loredana Guglielmi & Myrianni Constantinou & Nicola Pomella & Xinyu Zhang & James R. Boot & Tania A. Jones & Thomas O. Millner & Anaelle A. Dumas & Vardhman Rakyan & Je, 2021. "Comparative epigenetic analysis of tumour initiating cells and syngeneic EPSC-derived neural stem cells in glioblastoma," Nature Communications, Nature, vol. 12(1), pages 1-20, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-03190-3. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.