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Precise control of SCRaMbLE in synthetic haploid and diploid yeast

Author

Listed:
  • Bin Jia

    (Tianjin University
    Collaborative Innovation Center of Chemical Science and Engineering (Tianjin))

  • Yi Wu

    (Tianjin University
    Collaborative Innovation Center of Chemical Science and Engineering (Tianjin))

  • Bing-Zhi Li

    (Tianjin University
    Collaborative Innovation Center of Chemical Science and Engineering (Tianjin))

  • Leslie A. Mitchell

    (New York University Langone Medical Center)

  • Hong Liu

    (Tianjin University
    Collaborative Innovation Center of Chemical Science and Engineering (Tianjin))

  • Shuo Pan

    (Tianjin University
    Collaborative Innovation Center of Chemical Science and Engineering (Tianjin))

  • Juan Wang

    (Tianjin University
    Collaborative Innovation Center of Chemical Science and Engineering (Tianjin))

  • Hao-Ran Zhang

    (Tianjin University
    Collaborative Innovation Center of Chemical Science and Engineering (Tianjin))

  • Nan Jia

    (Tianjin University
    Collaborative Innovation Center of Chemical Science and Engineering (Tianjin))

  • Bo Li

    (Tianjin University
    Collaborative Innovation Center of Chemical Science and Engineering (Tianjin))

  • Michael Shen

    (New York University Langone Medical Center)

  • Ze-Xiong Xie

    (Tianjin University
    Collaborative Innovation Center of Chemical Science and Engineering (Tianjin))

  • Duo Liu

    (Tianjin University
    Collaborative Innovation Center of Chemical Science and Engineering (Tianjin))

  • Ying-Xiu Cao

    (Tianjin University
    Collaborative Innovation Center of Chemical Science and Engineering (Tianjin))

  • Xia Li

    (Tianjin University
    Collaborative Innovation Center of Chemical Science and Engineering (Tianjin))

  • Xiao Zhou

    (Tianjin University
    Collaborative Innovation Center of Chemical Science and Engineering (Tianjin))

  • Hao Qi

    (Tianjin University
    Collaborative Innovation Center of Chemical Science and Engineering (Tianjin))

  • Jef D. Boeke

    (New York University Langone Medical Center)

  • Ying-Jin Yuan

    (Tianjin University
    Collaborative Innovation Center of Chemical Science and Engineering (Tianjin))

Abstract

Compatibility between host cells and heterologous pathways is a challenge for constructing organisms with high productivity or gain of function. Designer yeast cells incorporating the Synthetic Chromosome Rearrangement and Modification by LoxP-mediated Evolution (SCRaMbLE) system provide a platform for generating genotype diversity. Here we construct a genetic AND gate to enable precise control of the SCRaMbLE method to generate synthetic haploid and diploid yeast with desired phenotypes. The yield of carotenoids is increased to 1.5-fold by SCRaMbLEing haploid strains and we determine that the deletion of YEL013W is responsible for the increase. Based on the SCRaMbLEing in diploid strains, we develop a strategy called Multiplex SCRaMbLE Iterative Cycling (MuSIC) to increase the production of carotenoids up to 38.8-fold through 5 iterative cycles of SCRaMbLE. This strategy is potentially a powerful tool for increasing the production of bio-based chemicals and for mining deep knowledge.

Suggested Citation

  • Bin Jia & Yi Wu & Bing-Zhi Li & Leslie A. Mitchell & Hong Liu & Shuo Pan & Juan Wang & Hao-Ran Zhang & Nan Jia & Bo Li & Michael Shen & Ze-Xiong Xie & Duo Liu & Ying-Xiu Cao & Xia Li & Xiao Zhou & Hao, 2018. "Precise control of SCRaMbLE in synthetic haploid and diploid yeast," Nature Communications, Nature, vol. 9(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-03084-4
    DOI: 10.1038/s41467-018-03084-4
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