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Basal condensation of Numb and Pon complex via phase transition during Drosophila neuroblast asymmetric division

Author

Listed:
  • Zelin Shan

    (Shanghai Medical College of Fudan University)

  • Yuting Tu

    (Shanghai Medical College of Fudan University)

  • Ying Yang

    (National University of Singapore)

  • Ziheng Liu

    (Shanghai Medical College of Fudan University)

  • Menglong Zeng

    (Hong Kong University of Science and Technology)

  • Huisha Xu

    (Nankai University)

  • Jiafu Long

    (Nankai University)

  • Mingjie Zhang

    (Hong Kong University of Science and Technology)

  • Yu Cai

    (National University of Singapore)

  • Wenyu Wen

    (Shanghai Medical College of Fudan University)

Abstract

Uneven distribution and local concentration of protein complexes on distinct membrane cortices is a fundamental property in numerous biological processes, including Drosophila neuroblast (NB) asymmetric cell divisions and cell polarity in general. In NBs, the cell fate determinant Numb forms a basal crescent together with Pon and is segregated into the basal daughter cell to initiate its differentiation. Here we discover that Numb PTB domain, using two distinct binding surfaces, recognizes repeating motifs within Pon in a previously unrecognized mode. The multivalent Numb-Pon interaction leads to high binding specificity and liquid-liquid phase separation of the complex. Perturbations of the Numb/Pon complex phase transition impair the basal localization of Numb and its subsequent suppression of Notch signaling during NB asymmetric divisions. Such phase-transition-mediated protein condensations on distinct membrane cortices may be a general mechanism for various cell polarity regulatory complexes.

Suggested Citation

  • Zelin Shan & Yuting Tu & Ying Yang & Ziheng Liu & Menglong Zeng & Huisha Xu & Jiafu Long & Mingjie Zhang & Yu Cai & Wenyu Wen, 2018. "Basal condensation of Numb and Pon complex via phase transition during Drosophila neuroblast asymmetric division," Nature Communications, Nature, vol. 9(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-03077-3
    DOI: 10.1038/s41467-018-03077-3
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    Cited by:

    1. Huanqing Cui & Yage Zhang & Sihan Liu & Yang Cao & Qingming Ma & Yuan Liu & Haisong Lin & Chang Li & Yang Xiao & Sammer Ul Hassan & Ho Cheung Shum, 2024. "Thermo-responsive aqueous two-phase system for two-level compartmentalization," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

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