IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v9y2018i1d10.1038_s41467-018-03061-x.html
   My bibliography  Save this article

A human endothelial cell-based recycling assay for screening of FcRn targeted molecules

Author

Listed:
  • Algirdas Grevys

    (University of Oslo
    Rikshospitalet, Oslo University Hospital and University of Oslo)

  • Jeannette Nilsen

    (Rikshospitalet, Oslo University Hospital and University of Oslo
    University of Oslo)

  • Kine M. K. Sand

    (University of Oslo
    Rikshospitalet, Oslo University Hospital and University of Oslo)

  • Muluneh B. Daba

    (University of Oslo
    Rikshospitalet, Oslo University Hospital and University of Oslo)

  • Inger Øynebråten

    (Oslo University Hospital and University of Oslo)

  • Malin Bern

    (University of Oslo
    Rikshospitalet, Oslo University Hospital and University of Oslo)

  • Martin B. McAdam

    (University of Oslo
    Rikshospitalet, Oslo University Hospital and University of Oslo)

  • Stian Foss

    (University of Oslo
    Rikshospitalet, Oslo University Hospital and University of Oslo)

  • Tilman Schlothauer

    (Roche Innovation Center)

  • Terje E. Michaelsen

    (University of Oslo
    Norwegian Institute of Public Health, Infection Immunology)

  • Gregory J. Christianson

    (Jackson Laboratory)

  • Derry C. Roopenian

    (Jackson Laboratory)

  • Richard S. Blumberg

    (Harvard Medical School)

  • Inger Sandlie

    (University of Oslo
    Rikshospitalet, Oslo University Hospital and University of Oslo)

  • Jan Terje Andersen

    (University of Oslo
    Rikshospitalet, Oslo University Hospital and University of Oslo
    University of Oslo and Oslo University Hospital)

Abstract

Albumin and IgG have remarkably long serum half-lives due to pH-dependent FcRn-mediated cellular recycling that rescues both ligands from intracellular degradation. Furthermore, increase in half-lives of IgG and albumin-based therapeutics has the potential to improve their efficacies, but there is a great need for robust methods for screening of relative FcRn-dependent recycling ability. Here, we report on a novel human endothelial cell-based recycling assay (HERA) that can be used for such pre-clinical screening. In HERA, rescue from degradation depends on FcRn, and engineered ligands are recycled in a manner that correlates with their half-lives in human FcRn transgenic mice. Thus, HERA is a novel cellular assay that can be used to predict how FcRn-binding proteins are rescued from intracellular degradation.

Suggested Citation

  • Algirdas Grevys & Jeannette Nilsen & Kine M. K. Sand & Muluneh B. Daba & Inger Øynebråten & Malin Bern & Martin B. McAdam & Stian Foss & Tilman Schlothauer & Terje E. Michaelsen & Gregory J. Christian, 2018. "A human endothelial cell-based recycling assay for screening of FcRn targeted molecules," Nature Communications, Nature, vol. 9(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-03061-x
    DOI: 10.1038/s41467-018-03061-x
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-018-03061-x
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-018-03061-x?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Torleif Tollefsrud Gjølberg & Jonas Aakre Wik & Hanna Johannessen & Stig Krüger & Nicola Bassi & Panagiotis F. Christopoulos & Malin Bern & Stian Foss & Goran Petrovski & Morten C. Moe & Guttorm Haral, 2023. "Antibody blockade of Jagged1 attenuates choroidal neovascularization," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    2. Stian Foss & Siri A. Sakya & Leire Aguinagalde & Marta Lustig & Jutamas Shaughnessy & Ana Rita Cruz & Lisette Scheepmaker & Line Mathiesen & Fulgencio Ruso-Julve & Aina Karen Anthi & Torleif Tollefsru, 2024. "Human IgG Fc-engineering for enhanced plasma half-life, mucosal distribution and killing of cancer cells and bacteria," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    3. Maximilian Brinkhaus & Erwin Pannecoucke & Elvera J. Kooi & Arthur E. H. Bentlage & Ninotska I. L. Derksen & Julie Andries & Bianca Balbino & Magdalena Sips & Peter Ulrichts & Peter Verheesen & Hans H, 2022. "The Fab region of IgG impairs the internalization pathway of FcRn upon Fc engagement," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-03061-x. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.