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A conserved ankyrin repeat-containing protein regulates conoid stability, motility and cell invasion in Toxoplasma gondii

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  • Shaojun Long

    (Washington University School of Medicine)

  • Bryan Anthony

    (Washington University School of Medicine)

  • Lisa L. Drewry

    (Washington University School of Medicine)

  • L. David Sibley

    (Washington University School of Medicine)

Abstract

Apicomplexan parasites are typified by an apical complex that contains a unique microtubule-organizing center (MTOC) that organizes the cytoskeleton. In apicomplexan parasites such as Toxoplasma gondii, the apical complex includes a spiral cap of tubulin-rich fibers called the conoid. Although described ultrastructurally, the composition and functions of the conoid are largely unknown. Here, we localize 11 previously undescribed apical proteins in T. gondii and identify an essential component named conoid protein hub 1 (CPH1), which is conserved in apicomplexan parasites. CPH1 contains ankyrin repeats that are required for structural integrity of the conoid, parasite motility, and host cell invasion. Proximity labeling and protein interaction network analysis reveal that CPH1 functions as a hub linking key motor and structural proteins that contain intrinsically disordered regions and coiled coil domains. Our findings highlight the importance of essential protein hubs in controlling biological networks of MTOCs in early-branching protozoan parasites.

Suggested Citation

  • Shaojun Long & Bryan Anthony & Lisa L. Drewry & L. David Sibley, 2017. "A conserved ankyrin repeat-containing protein regulates conoid stability, motility and cell invasion in Toxoplasma gondii," Nature Communications, Nature, vol. 8(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-02341-2
    DOI: 10.1038/s41467-017-02341-2
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    Cited by:

    1. Jin-Lei Wang & Ting-Ting Li & Hany M. Elsheikha & Qin-Li Liang & Zhi-Wei Zhang & Meng Wang & L. David Sibley & Xing-Quan Zhu, 2022. "The protein phosphatase 2A holoenzyme is a key regulator of starch metabolism and bradyzoite differentiation in Toxoplasma gondii," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    2. Bing Liu & Cong Liu & Zhenkui Li & Wenjia Liu & Huiting Cui & Jing Yuan, 2024. "A subpellicular microtubule dynein transport machinery regulates ookinete morphogenesis for mosquito transmission of Plasmodium yoelii," Nature Communications, Nature, vol. 15(1), pages 1-21, December.
    3. Long Gui & William J. O’Shaughnessy & Kai Cai & Evan Reetz & Michael L. Reese & Daniela Nicastro, 2023. "Cryo-tomography reveals rigid-body motion and organization of apicomplexan invasion machinery," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    4. Penelope L. Lindsay & Sergey Ivanov & Nathan Pumplin & Xinchun Zhang & Maria J. Harrison, 2022. "Distinct ankyrin repeat subdomains control VAPYRIN locations and intracellular accommodation functions during arbuscular mycorrhizal symbiosis," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    5. Wenyan Wan & Hui Dong & De-Hua Lai & Jiong Yang & Kai He & Xiaoyan Tang & Qun Liu & Geoff Hide & Xing-Quan Zhu & L. David Sibley & Zhao-Rong Lun & Shaojun Long, 2023. "The Toxoplasma micropore mediates endocytosis for selective nutrient salvage from host cell compartments," Nature Communications, Nature, vol. 14(1), pages 1-21, December.

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