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Stochastic gene expression in Arabidopsis thaliana

Author

Listed:
  • Ilka Schultheiß Araújo

    (Cologne University)

  • Jessica Magdalena Pietsch

    (Cologne University)

  • Emma Mathilde Keizer

    (Wageningen University)

  • Bettina Greese

    (Faculty for Theoretical Physics and Astronomy, Lund University)

  • Rachappa Balkunde

    (Cologne University)

  • Christian Fleck

    (Wageningen University)

  • Martin Hülskamp

    (Cologne University)

Abstract

Although plant development is highly reproducible, some stochasticity exists. This developmental stochasticity may be caused by noisy gene expression. Here we analyze the fluctuation of protein expression in Arabidopsis thaliana. Using the photoconvertible KikGR marker, we show that the protein expressions of individual cells fluctuate over time. A dual reporter system was used to study extrinsic and intrinsic noise of marker gene expression. We report that extrinsic noise is higher than intrinsic noise and that extrinsic noise in stomata is clearly lower in comparison to several other tissues/cell types. Finally, we show that cells are coupled with respect to stochastic protein expression in young leaves, hypocotyls and roots but not in mature leaves. Our data indicate that stochasticity of gene expression can vary between tissues/cell types and that it can be coupled in a non-cell-autonomous manner.

Suggested Citation

  • Ilka Schultheiß Araújo & Jessica Magdalena Pietsch & Emma Mathilde Keizer & Bettina Greese & Rachappa Balkunde & Christian Fleck & Martin Hülskamp, 2017. "Stochastic gene expression in Arabidopsis thaliana," Nature Communications, Nature, vol. 8(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-02285-7
    DOI: 10.1038/s41467-017-02285-7
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    Cited by:

    1. Mark Greenwood & Mirela Domijan & Peter D Gould & Anthony J W Hall & James C W Locke, 2019. "Coordinated circadian timing through the integration of local inputs in Arabidopsis thaliana," PLOS Biology, Public Library of Science, vol. 17(8), pages 1-31, August.

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