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Bactofilin-mediated organization of the ParABS chromosome segregation system in Myxococcus xanthus

Author

Listed:
  • Lin Lin

    (Philipps University
    Max Planck Institute for Terrestrial Microbiology
    University of Basel)

  • Manuel Osorio Valeriano

    (Philipps University
    Max Planck Institute for Terrestrial Microbiology)

  • Andrea Harms

    (Max Planck Institute for Terrestrial Microbiology)

  • Lotte Søgaard-Andersen

    (Max Planck Institute for Terrestrial Microbiology
    Hans-Meerwein-Straße)

  • Martin Thanbichler

    (Philipps University
    Max Planck Institute for Terrestrial Microbiology
    Hans-Meerwein-Straße)

Abstract

In bacteria, homologs of actin, tubulin, and intermediate filament proteins often act in concert with bacteria-specific scaffolding proteins to ensure the proper arrangement of cellular components. Among the bacteria-specific factors are the bactofilins, a widespread family of polymer-forming proteins whose biology is poorly investigated. Here, we study the three bactofilins BacNOP in the rod-shaped bacterium Myxococcus xanthus. We show that BacNOP co-assemble into elongated scaffolds that restrain the ParABS chromosome segregation machinery to the subpolar regions of the cell. The centromere (parS)-binding protein ParB associates with the pole-distal ends of these structures, whereas the DNA partitioning ATPase ParA binds along their entire length, using the newly identified protein PadC (MXAN_4634) as an adapter. The integrity of these complexes is critical for proper nucleoid morphology and chromosome segregation. BacNOP thus mediate a previously unknown mechanism of subcellular organization that recruits proteins to defined sites within the cytoplasm, far off the cell poles.

Suggested Citation

  • Lin Lin & Manuel Osorio Valeriano & Andrea Harms & Lotte Søgaard-Andersen & Martin Thanbichler, 2017. "Bactofilin-mediated organization of the ParABS chromosome segregation system in Myxococcus xanthus," Nature Communications, Nature, vol. 8(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-02015-z
    DOI: 10.1038/s41467-017-02015-z
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    Cited by:

    1. Ismath Sadhir & Seán M. Murray, 2023. "Mid-cell migration of the chromosomal terminus is coupled to origin segregation in Escherichia coli," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    2. Lavisha Jindal & Eldon Emberly, 2019. "DNA segregation under Par protein control," PLOS ONE, Public Library of Science, vol. 14(7), pages 1-20, July.

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