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She1 affects dynein through direct interactions with the microtubule and the dynein microtubule-binding domain

Author

Listed:
  • Kari H. Ecklund

    (Colorado State University)

  • Tatsuya Morisaki

    (Colorado State University)

  • Lindsay G. Lammers

    (Colorado State University)

  • Matthew G. Marzo

    (Colorado State University)

  • Timothy J. Stasevich

    (Colorado State University)

  • Steven M. Markus

    (Colorado State University)

Abstract

Cytoplasmic dynein is an enormous minus end-directed microtubule motor. Rather than existing as bare tracks, microtubules are bound by numerous microtubule-associated proteins (MAPs) that have the capacity to affect various cellular functions, including motor-mediated transport. One such MAP is She1, a dynein effector that polarizes dynein-mediated spindle movements in budding yeast. Here, we characterize the molecular basis by which She1 affects dynein, providing the first such insight into which a MAP can modulate motor motility. We find that She1 affects the ATPase rate, microtubule-binding affinity, and stepping behavior of dynein, and that microtubule binding by She1 is required for its effects on dynein motility. Moreover, we find that She1 directly contacts the microtubule-binding domain of dynein, and that their interaction is sensitive to the nucleotide-bound state of the motor. Our data support a model in which simultaneous interactions between the microtubule and dynein enables She1 to directly affect dynein motility.

Suggested Citation

  • Kari H. Ecklund & Tatsuya Morisaki & Lindsay G. Lammers & Matthew G. Marzo & Timothy J. Stasevich & Steven M. Markus, 2017. "She1 affects dynein through direct interactions with the microtubule and the dynein microtubule-binding domain," Nature Communications, Nature, vol. 8(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-02004-2
    DOI: 10.1038/s41467-017-02004-2
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    Cited by:

    1. Kyoko Okada & Bharat R. Iyer & Lindsay G. Lammers & Pedro A. Gutierrez & Wenzhe Li & Steven M. Markus & Richard J. McKenney, 2023. "Conserved roles for the dynein intermediate chain and Ndel1 in assembly and activation of dynein," Nature Communications, Nature, vol. 14(1), pages 1-19, December.

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