IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v8y2017i1d10.1038_s41467-017-01946-x.html
   My bibliography  Save this article

Cardiopatch platform enables maturation and scale-up of human pluripotent stem cell-derived engineered heart tissues

Author

Listed:
  • Ilya Y. Shadrin

    (Duke University)

  • Brian W. Allen

    (Duke University)

  • Ying Qian

    (Duke University)

  • Christopher P. Jackman

    (Duke University)

  • Aaron L. Carlson

    (Duke University)

  • Mark E. Juhas

    (Duke University)

  • Nenad Bursac

    (Duke University)

Abstract

Despite increased use of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) for drug development and disease modeling studies, methods to generate large, functional heart tissues for human therapy are lacking. Here we present a “Cardiopatch” platform for 3D culture and maturation of hiPSC-CMs that after 5 weeks of differentiation show robust electromechanical coupling, consistent H-zones, I-bands, and evidence for T-tubules and M-bands. Cardiopatch maturation markers and functional output increase during culture, approaching values of adult myocardium. Cardiopatches can be scaled up to clinically relevant dimensions, while preserving spatially uniform properties with high conduction velocities and contractile stresses. Within window chambers in nude mice, cardiopatches undergo vascularization by host vessels and continue to fire Ca2+ transients. When implanted onto rat hearts, cardiopatches robustly engraft, maintain pre-implantation electrical function, and do not increase the incidence of arrhythmias. These studies provide enabling technology for future use of hiPSC-CM tissues in human heart repair.

Suggested Citation

  • Ilya Y. Shadrin & Brian W. Allen & Ying Qian & Christopher P. Jackman & Aaron L. Carlson & Mark E. Juhas & Nenad Bursac, 2017. "Cardiopatch platform enables maturation and scale-up of human pluripotent stem cell-derived engineered heart tissues," Nature Communications, Nature, vol. 8(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01946-x
    DOI: 10.1038/s41467-017-01946-x
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-017-01946-x
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-017-01946-x?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Mao Mao & Xiaoli Qu & Yabo Zhang & Bingsong Gu & Chen Li & Rongzhi Liu & Xiao Li & Hui Zhu & Jiankang He & Dichen Li, 2023. "Leaf-venation-directed cellular alignment for macroscale cardiac constructs with tissue-like functionalities," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    2. Yutong He & Qian Li & Pinger Chen & Qixiang Duan & Jiamian Zhan & Xiaohui Cai & Leyu Wang & Honghao Hou & Xiaozhong Qiu, 2022. "A smart adhesive Janus hydrogel for non-invasive cardiac repair and tissue adhesion prevention," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
    3. Renjie Qiu & Xingying Zhang & Chen Song & Kaige Xu & Huijia Nong & Yi Li & Xianglong Xing & Kibret Mequanint & Qian Liu & Quan Yuan & Xiaomin Sun & Malcolm Xing & Leyu Wang, 2024. "E-cardiac patch to sense and repair infarcted myocardium," Nature Communications, Nature, vol. 15(1), pages 1-20, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01946-x. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.