IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v8y2017i1d10.1038_s41467-017-01876-8.html
   My bibliography  Save this article

Coupling of Polo kinase activation to nuclear localization by a bifunctional NLS is required during mitotic entry

Author

Listed:
  • David Kachaner

    (Institute for Research in Immunology and Cancer, Université de Montréal, C.P. 6128 Succursale Centre-Ville
    Université de Montréal, C.P. 6128 Succursale Centre-Ville)

  • Damien Garrido

    (Institute for Research in Immunology and Cancer, Université de Montréal, C.P. 6128 Succursale Centre-Ville
    Université de Montréal, C.P. 6128 Succursale Centre-Ville)

  • Haytham Mehsen

    (Institute for Research in Immunology and Cancer, Université de Montréal, C.P. 6128 Succursale Centre-Ville)

  • Karine Normandin

    (Institute for Research in Immunology and Cancer, Université de Montréal, C.P. 6128 Succursale Centre-Ville)

  • Hugo Lavoie

    (Institute for Research in Immunology and Cancer, Université de Montréal, C.P. 6128 Succursale Centre-Ville)

  • Vincent Archambault

    (Institute for Research in Immunology and Cancer, Université de Montréal, C.P. 6128 Succursale Centre-Ville
    Université de Montréal, C.P. 6128 Succursale Centre-Ville)

Abstract

The Polo kinase is a master regulator of mitosis and cytokinesis conserved from yeasts to humans. Polo is composed of an N-term kinase domain (KD) and a C-term polo-box domain (PBD), which regulates its subcellular localizations. The PBD and KD can interact and inhibit each other, and this reciprocal inhibition is relieved when Polo is phosphorylated at its activation loop. How Polo activation and localization are coupled during mitotic entry is unknown. Here we report that PBD binding to the KD masks a nuclear localization signal (NLS). Activating phosphorylation of the KD leads to exposure of the NLS and entry of Polo into the nucleus before nuclear envelope breakdown. Failures of this mechanism result in misregulation of the Cdk1-activating Cdc25 phosphatase and lead to mitotic and developmental defects in Drosophila. These results uncover spatiotemporal mechanisms linking master regulatory enzymes during mitotic entry.

Suggested Citation

  • David Kachaner & Damien Garrido & Haytham Mehsen & Karine Normandin & Hugo Lavoie & Vincent Archambault, 2017. "Coupling of Polo kinase activation to nuclear localization by a bifunctional NLS is required during mitotic entry," Nature Communications, Nature, vol. 8(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01876-8
    DOI: 10.1038/s41467-017-01876-8
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-017-01876-8
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-017-01876-8?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Shuo Yang & Jennifer McAdow & Yingqiu Du & Jennifer Trigg & Paul H. Taghert & Aaron N. Johnson, 2022. "Spatiotemporal expression of regulatory kinases directs the transition from mitosis to cellular morphogenesis in Drosophila," Nature Communications, Nature, vol. 13(1), pages 1-16, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01876-8. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.