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The semiquinone swing in the bifurcating electron transferring flavoprotein/butyryl-CoA dehydrogenase complex from Clostridium difficile

Author

Listed:
  • Julius K. Demmer

    (Max-Planck-Institut für Biophysik)

  • Nilanjan Pal Chowdhury

    (Laboratorium für Mikrobiologie, Fachbereich Biologie and SYNMIKRO, Philipps-Universität
    Max-Planck-Institut für terrestrische Mikrobiologie)

  • Thorsten Selmer

    (Fachbereich Chemie und Biotechnologie, FH Aachen)

  • Ulrich Ermler

    (Max-Planck-Institut für Biophysik)

  • Wolfgang Buckel

    (Laboratorium für Mikrobiologie, Fachbereich Biologie and SYNMIKRO, Philipps-Universität
    Max-Planck-Institut für terrestrische Mikrobiologie)

Abstract

The electron transferring flavoprotein/butyryl-CoA dehydrogenase (EtfAB/Bcd) catalyzes the reduction of one crotonyl-CoA and two ferredoxins by two NADH within a flavin-based electron-bifurcating process. Here we report on the X-ray structure of the Clostridium difficile (EtfAB/Bcd)4 complex in the dehydrogenase-conducting D-state, α-FAD (bound to domain II of EtfA) and δ-FAD (bound to Bcd) being 8 Å apart. Superimposing Acidaminococcus fermentans EtfAB onto C. difficile EtfAB/Bcd reveals a rotation of domain II of nearly 80°. Further rotation by 10° brings EtfAB into the bifurcating B-state, α-FAD and β-FAD (bound to EtfB) being 14 Å apart. This dual binding mode of domain II, substantiated by mutational studies, resembles findings in non-bifurcating EtfAB/acyl-CoA dehydrogenase complexes. In our proposed mechanism, NADH reduces β-FAD, which bifurcates. One electron goes to ferredoxin and one to α-FAD, which swings over to reduce δ-FAD to the semiquinone. Repetition affords a second reduced ferredoxin and δ-FADH−, which reduces crotonyl-CoA.

Suggested Citation

  • Julius K. Demmer & Nilanjan Pal Chowdhury & Thorsten Selmer & Ulrich Ermler & Wolfgang Buckel, 2017. "The semiquinone swing in the bifurcating electron transferring flavoprotein/butyryl-CoA dehydrogenase complex from Clostridium difficile," Nature Communications, Nature, vol. 8(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01746-3
    DOI: 10.1038/s41467-017-01746-3
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    Cited by:

    1. Stella Vitt & Simone Prinz & Martin Eisinger & Ulrich Ermler & Wolfgang Buckel, 2022. "Purification and structural characterization of the Na+-translocating ferredoxin: NAD+ reductase (Rnf) complex of Clostridium tetanomorphum," Nature Communications, Nature, vol. 13(1), pages 1-11, December.

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