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Pericytes regulate VEGF-induced endothelial sprouting through VEGFR1

Author

Listed:
  • Hanna M. Eilken

    (Faculty of Medicine
    Bayer AG)

  • Rodrigo Diéguez-Hurtado

    (Faculty of Medicine)

  • Inga Schmidt

    (Faculty of Medicine)

  • Masanori Nakayama

    (Faculty of Medicine
    Max Planck Institute for Heart and Lung Research, Laboratory for Cell Polarity and Organogenesis)

  • Hyun-Woo Jeong

    (Faculty of Medicine)

  • Hendrik Arf

    (Faculty of Medicine)

  • Susanne Adams

    (Faculty of Medicine)

  • Napoleone Ferrara

    (University of California San Diego Medical Center)

  • Ralf H. Adams

    (Faculty of Medicine)

Abstract

Pericytes adhere to the abluminal surface of endothelial tubules and are required for the formation of stable vascular networks. Defective endothelial cell-pericyte interactions are frequently observed in diseases characterized by compromised vascular integrity such as diabetic retinopathy. Many functional properties of pericytes and their exact role in the regulation of angiogenic blood vessel growth remain elusive. Here we show that pericytes promote endothelial sprouting in the postnatal retinal vasculature. Using genetic and pharmacological approaches, we show that the expression of vascular endothelial growth factor receptor 1 (VEGFR1) by pericytes spatially restricts VEGF signalling. Angiogenic defects caused by pericyte depletion are phenocopied by intraocular injection of VEGF-A or pericyte-specific inactivation of the murine gene encoding VEGFR1. Our findings establish that pericytes promote endothelial sprouting, which results in the loss of side branches and the enlargement of vessels when pericyte function is impaired or lost.

Suggested Citation

  • Hanna M. Eilken & Rodrigo Diéguez-Hurtado & Inga Schmidt & Masanori Nakayama & Hyun-Woo Jeong & Hendrik Arf & Susanne Adams & Napoleone Ferrara & Ralf H. Adams, 2017. "Pericytes regulate VEGF-induced endothelial sprouting through VEGFR1," Nature Communications, Nature, vol. 8(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01738-3
    DOI: 10.1038/s41467-017-01738-3
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    Cited by:

    1. Manas Pratim Chakraborty & Diptatanu Das & Purav Mondal & Pragya Kaul & Soumi Bhattacharyya & Prosad Kumar Das & Rahul Das, 2024. "Molecular basis of VEGFR1 autoinhibition at the plasma membrane," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

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