IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v8y2017i1d10.1038_s41467-017-01668-0.html
   My bibliography  Save this article

Unique architecture of thermophilic archaeal virus APBV1 and its genome packaging

Author

Listed:
  • Denis Ptchelkine

    (University of Oxford
    Rutherford Appleton Laboratory)

  • Ashley Gillum

    (University of Oxford)

  • Tomohiro Mochizuki

    (Institut Pasteur
    Tokyo Institute of Technology)

  • Soizick Lucas-Staat

    (Institut Pasteur)

  • Ying Liu

    (Institut Pasteur)

  • Mart Krupovic

    (Institut Pasteur)

  • Simon E. V. Phillips

    (Rutherford Appleton Laboratory
    University of Oxford)

  • David Prangishvili

    (Institut Pasteur)

  • Juha T. Huiskonen

    (University of Oxford
    University of Helsinki)

Abstract

Archaeal viruses have evolved to infect hosts often thriving in extreme conditions such as high temperatures. However, there is a paucity of information on archaeal virion structures, genome packaging, and determinants of temperature resistance. The rod-shaped virus APBV1 (Aeropyrum pernix bacilliform virus 1) is among the most thermostable viruses known; it infects a hyperthermophile Aeropyrum pernix, which grows optimally at 90 °C. Here we report the structure of APBV1, determined by cryo-electron microscopy at near-atomic resolution. Tight packing of the major virion glycoprotein (VP1) is ensured by extended hydrophobic interfaces, and likely contributes to the extreme thermostability of the helical capsid. The double-stranded DNA is tightly packed in the capsid as a left-handed superhelix and held in place by the interactions with positively charged residues of VP1. The assembly is closed by specific capping structures at either end, which we propose to play a role in DNA packing and delivery.

Suggested Citation

  • Denis Ptchelkine & Ashley Gillum & Tomohiro Mochizuki & Soizick Lucas-Staat & Ying Liu & Mart Krupovic & Simon E. V. Phillips & David Prangishvili & Juha T. Huiskonen, 2017. "Unique architecture of thermophilic archaeal virus APBV1 and its genome packaging," Nature Communications, Nature, vol. 8(1), pages 1-6, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01668-0
    DOI: 10.1038/s41467-017-01668-0
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-017-01668-0
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-017-01668-0?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01668-0. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.