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Reassessment of chitosanase substrate specificities and classification

Author

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  • Tobias Weikert

    (Institute for Biology and Biotechnology of Plants, University of Münster)

  • Anna Niehues

    (Institute for Biology and Biotechnology of Plants, University of Münster)

  • Stefan Cord-Landwehr

    (Institute for Biology and Biotechnology of Plants, University of Münster)

  • Margareta J. Hellmann

    (Institute for Biology and Biotechnology of Plants, University of Münster)

  • Bruno M. Moerschbacher

    (Institute for Biology and Biotechnology of Plants, University of Münster)

Abstract

Chitosanases can be used to produce partially acetylated chitosan oligosaccharides (paCOS) for different applications, provided they are thoroughly characterized. However, recent studies indicate that the established classification system for chitosanases is too simplistic. Here, we apply a highly sensitive method for quantitatively sequencing paCOS to reassess the substrate specificities of the best-characterized class I–III chitosanases. The enzymes’ abilities to cleave bonds at GlcNAc residues positioned at subsite (−1) or (+1), on which the classification system is based, vary especially when the substrates have different fractions of acetylation (F A ). Conflicts with the recent classification are observed at higher F A , which were not investigated in prior specificity determinations. Initial analyses of pectin-degrading enzymes reveal that classifications of other polysaccharide-degrading enzymes should also be critically reassessed. Based on our results, we tentatively suggest a chitosanase classification system which is based on specificities and preferences of subsites (−2) to (+2).

Suggested Citation

  • Tobias Weikert & Anna Niehues & Stefan Cord-Landwehr & Margareta J. Hellmann & Bruno M. Moerschbacher, 2017. "Reassessment of chitosanase substrate specificities and classification," Nature Communications, Nature, vol. 8(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01667-1
    DOI: 10.1038/s41467-017-01667-1
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    Cited by:

    1. Kumari, Rajni & Kumar, Manish & Vivekanand, V. & Pareek, Nidhi, 2023. "Chitin biorefinery: A narrative and prophecy of crustacean shell waste sustainable transformation into bioactives and renewable energy," Renewable and Sustainable Energy Reviews, Elsevier, vol. 184(C).

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