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Label-free automated three-dimensional imaging of whole organs by microtomy-assisted photoacoustic microscopy

Author

Listed:
  • Terence T. W. Wong

    (California Institute of Technology
    Washington University in St. Louis)

  • Ruiying Zhang

    (Washington University in St. Louis)

  • Chi Zhang

    (Washington University in St. Louis)

  • Hsun-Chia Hsu

    (California Institute of Technology
    Washington University in St. Louis)

  • Konstantin I. Maslov

    (California Institute of Technology)

  • Lidai Wang

    (Washington University in St. Louis
    City University of Hong Kong)

  • Junhui Shi

    (California Institute of Technology)

  • Ruimin Chen

    (University of Southern California)

  • K. Kirk Shung

    (University of Southern California)

  • Qifa Zhou

    (University of Southern California
    University of Southern California)

  • Lihong V. Wang

    (California Institute of Technology)

Abstract

Three-dimensional (3D) optical imaging of whole biological organs with microscopic resolution has remained a challenge. Most versions of such imaging techniques require special preparation of the tissue specimen. Here we demonstrate microtomy-assisted photoacoustic microscopy (mPAM) of mouse brains and other organs, which automatically acquires serial distortion-free and registration-free images with endogenous absorption contrasts. Without tissue staining or clearing, mPAM generates micrometer-resolution 3D images of paraffin- or agarose-embedded whole organs with high fidelity, achieved by label-free simultaneous sensing of DNA/RNA, hemoglobins, and lipids. mPAM provides histology-like imaging of cell nuclei, blood vessels, axons, and other anatomical structures, enabling the application of histopathological interpretation at the organelle level to analyze a whole organ. Its deep tissue imaging capability leads to less sectioning, resulting in negligible sectioning artifact. mPAM offers a new way to better understand complex biological organs.

Suggested Citation

  • Terence T. W. Wong & Ruiying Zhang & Chi Zhang & Hsun-Chia Hsu & Konstantin I. Maslov & Lidai Wang & Junhui Shi & Ruimin Chen & K. Kirk Shung & Qifa Zhou & Lihong V. Wang, 2017. "Label-free automated three-dimensional imaging of whole organs by microtomy-assisted photoacoustic microscopy," Nature Communications, Nature, vol. 8(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01649-3
    DOI: 10.1038/s41467-017-01649-3
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