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Single molecule multiplexed nanopore protein screening in human serum using aptamer modified DNA carriers

Author

Listed:
  • Jasmine Y. Y. Sze

    (Imperial College London)

  • Aleksandar P. Ivanov

    (Imperial College London)

  • Anthony E. G. Cass

    (Imperial College London)

  • Joshua B. Edel

    (Imperial College London)

Abstract

The capability to screen a range of proteins at the single-molecule level with enhanced selectivity in biological fluids has been in part a driving force in developing future diagnostic and therapeutic strategies. The combination of nanopore sensing and nucleic acid aptamer recognition comes close to this ideal due to the ease of multiplexing, without the need for expensive labelling methods or extensive sample pre-treatment. Here, we demonstrate a fully flexible, scalable and low-cost detection platform to sense multiple protein targets simultaneously by grafting specific sequences along the backbone of a double-stranded DNA carrier. Protein bound to the aptamer produces unique ionic current signatures which facilitates accurate target recognition. This powerful approach allows us to differentiate individual protein sizes via characteristic changes in the sub-peak current. Furthermore, we show that by using DNA carriers it is possible to perform single-molecule screening in human serum at ultra-low protein concentrations.

Suggested Citation

  • Jasmine Y. Y. Sze & Aleksandar P. Ivanov & Anthony E. G. Cass & Joshua B. Edel, 2017. "Single molecule multiplexed nanopore protein screening in human serum using aptamer modified DNA carriers," Nature Communications, Nature, vol. 8(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01584-3
    DOI: 10.1038/s41467-017-01584-3
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    Cited by:

    1. Ren Ren & Shenglin Cai & Xiaona Fang & Xiaoyi Wang & Zheng Zhang & Micol Damiani & Charlotte Hudlerova & Annachiara Rosa & Joshua Hope & Nicola J. Cook & Peter Gorelkin & Alexander Erofeev & Pavel Nov, 2023. "Multiplexed detection of viral antigen and RNA using nanopore sensing and encoded molecular probes," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

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