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Influenza virus genome reaches the plasma membrane via a modified endoplasmic reticulum and Rab11-dependent vesicles

Author

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  • Isabel Fernández de Castro Martin

    (Centro Nacional de Biotecnologia, CNB-CSIC, Department of Macromolecular Structures, Cell Structure Lab)

  • Guillaume Fournier

    (Institut Pasteur, Unité de Génétique Moléculaire des Virus à ARN
    CNRS, UMR3569
    Université Paris Diderot, Sorbonne Paris Cité, EA302)

  • Martin Sachse

    (Center for Innovation and Technological Research, Ultrastructural Bio-imaging, Institut Pasteur)

  • Javier Pizarro-Cerda

    (Institut Pasteur, Unité des Interactions Bactéries-Cellules
    INSERM
    Institut Pasteur
    Centre National de Référence ‘Peste et autres Yersinioses’)

  • Cristina Risco

    (Centro Nacional de Biotecnologia, CNB-CSIC, Department of Macromolecular Structures, Cell Structure Lab)

  • Nadia Naffakh

    (Institut Pasteur, Unité de Génétique Moléculaire des Virus à ARN
    CNRS, UMR3569
    Université Paris Diderot, Sorbonne Paris Cité, EA302)

Abstract

Transport of neo-synthesized influenza A virus (IAV) viral ribonucleoproteins (vRNPs) from the nucleus to the plasma membrane involves Rab 11 but the precise mechanism remains poorly understood. We used metal-tagging and immunolabeling to visualize viral proteins and cellular endomembrane markers by electron microscopy of IAV-infected cells. Unexpectedly, we provide evidence that the vRNP components and the Rab11 protein are present at the membrane of a modified, tubulated endoplasmic reticulum (ER) that extends all throughout the cell, and on irregularly coated vesicles (ICVs). Some ICVs are found very close to the ER and to the plasma membrane. ICV formation is observed only in infected cells and requires an active Rab11 GTPase. Against the currently accepted model in which vRNPs are carried onto Rab11-positive recycling endosomes across the cytoplasm, our findings reveal that the endomembrane organelle that is primarily involved in the transport of vRNPs is the ER.

Suggested Citation

  • Isabel Fernández de Castro Martin & Guillaume Fournier & Martin Sachse & Javier Pizarro-Cerda & Cristina Risco & Nadia Naffakh, 2017. "Influenza virus genome reaches the plasma membrane via a modified endoplasmic reticulum and Rab11-dependent vesicles," Nature Communications, Nature, vol. 8(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01557-6
    DOI: 10.1038/s41467-017-01557-6
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    Cited by:

    1. Timothy D Majarian & Robert F Murphy & Seema S Lakdawala, 2019. "Learning the sequence of influenza A genome assembly during viral replication using point process models and fluorescence in situ hybridization," PLOS Computational Biology, Public Library of Science, vol. 15(1), pages 1-20, January.

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