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Chromosome segregation occurs by microtubule pushing in oocytes

Author

Listed:
  • Kimberley Laband

    (University Paris Diderot)

  • Rémi Le Borgne

    (University Paris Diderot)

  • Frances Edwards

    (University Paris Diderot)

  • Marine Stefanutti

    (University Paris Diderot)

  • Julie C. Canman

    (Columbia University Medical Center)

  • Jean-Marc Verbavatz

    (University Paris Diderot
    Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG))

  • Julien Dumont

    (University Paris Diderot)

Abstract

During cell division, spindle microtubules ensure an equal repartition of chromosomes between the two daughter cells. While the kinetochore-dependent mechanisms that drive mitotic chromosome segregation are well understood, in oocytes of most species atypical spindles assembled in absence of centrosomes entail poorly understood mechanisms of chromosome segregation. In particular, the structure(s) responsible for force generation during meiotic chromosome separation in oocytes is unclear. Using quantitative light microscopy, electron tomography, laser-mediated ablation, and genetic perturbations in the Caenorhabditis elegans oocyte, we studied the mechanism of chromosome segregation in meiosis. We find spindle poles are largely dispensable, and in fact act as brakes for chromosome segregation. Instead, our results suggest that CLS-2-dependent microtubules of the meiotic central spindle, located between the segregating chromosomes and aligned along the axis of segregation, are essential. Our results support a model in which inter-chromosomal microtubules of the central spindle push chromosomes apart during meiotic anaphase in oocytes.

Suggested Citation

  • Kimberley Laband & Rémi Le Borgne & Frances Edwards & Marine Stefanutti & Julie C. Canman & Jean-Marc Verbavatz & Julien Dumont, 2017. "Chromosome segregation occurs by microtubule pushing in oocytes," Nature Communications, Nature, vol. 8(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01539-8
    DOI: 10.1038/s41467-017-01539-8
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    Cited by:

    1. Laras Pitayu-Nugroho & Mélanie Aubry & Kimberley Laband & Hélène Geoffroy & Thadshagine Ganeswaran & Audi Primadhanty & Julie C. Canman & Julien Dumont, 2023. "Kinetochore component function in C. elegans oocytes revealed by 4D tracking of holocentric chromosomes," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

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