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Longitudinal intravital imaging of the femoral bone marrow reveals plasticity within marrow vasculature

Author

Listed:
  • David Reismann

    (A Leibniz Institute, Charitéplatz 1)

  • Jonathan Stefanowski

    (A Leibniz Institute, Charitéplatz 1
    Charité—Universitätsmedizin, Charitéplatz 1)

  • Robert Günther

    (A Leibniz Institute, Charitéplatz 1)

  • Asylkhan Rakhymzhan

    (A Leibniz Institute, Charitéplatz 1)

  • Romano Matthys

    (Talstraße 2A)

  • Reto Nützi

    (Talstraße 2A)

  • Sandra Zehentmeier

    (A Leibniz Institute, Charitéplatz 1
    Charité—Universitätsmedizin, Charitéplatz 1
    Yale University School of Medicine)

  • Katharina Schmidt-Bleek

    (Charité—Universitätsmedizin, Augustenburger Platz 1)

  • Georg Petkau

    (A Leibniz Institute, Charitéplatz 1)

  • Hyun-Dong Chang

    (A Leibniz Institute, Charitéplatz 1)

  • Sandra Naundorf

    (A Leibniz Institute, Charitéplatz 1)

  • York Winter

    (Unter den Linden 6)

  • Fritz Melchers

    (A Leibniz Institute, Charitéplatz 1)

  • Georg Duda

    (Charité—Universitätsmedizin, Augustenburger Platz 1)

  • Anja E. Hauser

    (A Leibniz Institute, Charitéplatz 1
    Charité—Universitätsmedizin, Charitéplatz 1)

  • Raluca A. Niesner

    (A Leibniz Institute, Charitéplatz 1)

Abstract

The bone marrow is a central organ of the immune system, which hosts complex interactions of bone and immune compartments critical for hematopoiesis, immunological memory, and bone regeneration. Although these processes take place over months, most existing imaging techniques allow us to follow snapshots of only a few hours, at subcellular resolution. Here, we develop a microendoscopic multi-photon imaging approach called LIMB (longitudinal intravital imaging of the bone marrow) to analyze cellular dynamics within the deep marrow. The approach consists of a biocompatible plate surgically fixated to the mouse femur containing a gradient refractive index lens. This microendoscope allows highly resolved imaging, repeatedly at the same regions within marrow tissue, over months. LIMB reveals extensive vascular plasticity during bone healing and steady-state homeostasis. To our knowledge, this vascular plasticity is unique among mammalian tissues, and we expect this insight will decisively change our understanding of essential phenomena occurring within the bone marrow.

Suggested Citation

  • David Reismann & Jonathan Stefanowski & Robert Günther & Asylkhan Rakhymzhan & Romano Matthys & Reto Nützi & Sandra Zehentmeier & Katharina Schmidt-Bleek & Georg Petkau & Hyun-Dong Chang & Sandra Naun, 2017. "Longitudinal intravital imaging of the femoral bone marrow reveals plasticity within marrow vasculature," Nature Communications, Nature, vol. 8(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01538-9
    DOI: 10.1038/s41467-017-01538-9
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    Cited by:

    1. M. Gabriele Bixel & Kishor K. Sivaraj & Melanie Timmen & Vishal Mohanakrishnan & Anusha Aravamudhan & Susanne Adams & Bong-Ihn Koh & Hyun-Woo Jeong & Kai Kruse & Richard Stange & Ralf H. Adams, 2024. "Angiogenesis is uncoupled from osteogenesis during calvarial bone regeneration," Nature Communications, Nature, vol. 15(1), pages 1-22, December.
    2. Till Fabian Mertens & Alina Tabea Liebheit & Johanna Ehl & Ralf Köhler & Asylkhan Rakhymzhan & Andrew Woehler & Lukas Katthän & Gernot Ebel & Wjatscheslaw Liublin & Ana Kasapi & Antigoni Triantafyllop, 2024. "MarShie: a clearing protocol for 3D analysis of single cells throughout the bone marrow at subcellular resolution," Nature Communications, Nature, vol. 15(1), pages 1-19, December.

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