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piRNAs and Aubergine cooperate with Wispy poly(A) polymerase to stabilize mRNAs in the germ plasm

Author

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  • Jérémy Dufourt

    (Institute of Human Genetics, UMR9002 CNRS-University of Montpellier)

  • Gwénaëlle Bontonou

    (Institute of Human Genetics, UMR9002 CNRS-University of Montpellier)

  • Aymeric Chartier

    (Institute of Human Genetics, UMR9002 CNRS-University of Montpellier)

  • Camille Jahan

    (Institute of Human Genetics, UMR9002 CNRS-University of Montpellier)

  • Anne-Cécile Meunier

    (Institute of Human Genetics, UMR9002 CNRS-University of Montpellier)

  • Stéphanie Pierson

    (Institute of Human Genetics, UMR9002 CNRS-University of Montpellier)

  • Paul F. Harrison

    (Monash University
    Monash Biomedicine Discovery Institute, Monash University)

  • Catherine Papin

    (Institute of Human Genetics, UMR9002 CNRS-University of Montpellier)

  • Traude H. Beilharz

    (Monash Biomedicine Discovery Institute, Monash University)

  • Martine Simonelig

    (Institute of Human Genetics, UMR9002 CNRS-University of Montpellier)

Abstract

Piwi-interacting RNAs (piRNAs) and PIWI proteins play a crucial role in germ cells by repressing transposable elements and regulating gene expression. In Drosophila, maternal piRNAs are loaded into the embryo mostly bound to the PIWI protein Aubergine (Aub). Aub targets maternal mRNAs through incomplete base-pairing with piRNAs and can induce their destabilization in the somatic part of the embryo. Paradoxically, these Aub-dependent unstable mRNAs encode germ cell determinants that are selectively stabilized in the germ plasm. Here we show that piRNAs and Aub actively protect germ cell mRNAs in the germ plasm. Aub directly interacts with the germline-specific poly(A) polymerase Wispy, thus leading to mRNA polyadenylation and stabilization in the germ plasm. These results reveal a role for piRNAs in mRNA stabilization and identify Aub as an interactor of Wispy for mRNA polyadenylation. They further highlight the role of Aub and piRNAs in embryonic patterning through two opposite functions.

Suggested Citation

  • Jérémy Dufourt & Gwénaëlle Bontonou & Aymeric Chartier & Camille Jahan & Anne-Cécile Meunier & Stéphanie Pierson & Paul F. Harrison & Catherine Papin & Traude H. Beilharz & Martine Simonelig, 2017. "piRNAs and Aubergine cooperate with Wispy poly(A) polymerase to stabilize mRNAs in the germ plasm," Nature Communications, Nature, vol. 8(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01431-5
    DOI: 10.1038/s41467-017-01431-5
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    Cited by:

    1. Anne Ramat & Ali Haidar & Céline Garret & Martine Simonelig, 2024. "Spatial organization of translation and translational repression in two phases of germ granules," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    2. Xavier Contreras & David Depierre & Charbel Akkawi & Marina Srbic & Marion Helsmoortel & Maguelone Nogaret & Matthieu LeHars & Kader Salifou & Alexandre Heurteau & Olivier Cuvier & Rosemary Kiernan, 2023. "PAPγ associates with PAXT nuclear exosome to control the abundance of PROMPT ncRNAs," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

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