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In vivo neurochemical measurements in cerebral tissues using a droplet-based monitoring system

Author

Listed:
  • Guillaume Petit-Pierre

    (Laboratory of Microsystems LMIS4, Ecole Polytechnique Fédérale de Lausanne (EPFL))

  • Philippe Colin

    (Brain Mind Institute, Ecole Polytechnique Fédérale de Lausanne (EPFL))

  • Estelle Laurer

    (Lausanne University Hospital, Geneva University Hospitals)

  • Julien Déglon

    (Lausanne University Hospital, Geneva University Hospitals)

  • Arnaud Bertsch

    (Laboratory of Microsystems LMIS4, Ecole Polytechnique Fédérale de Lausanne (EPFL))

  • Aurélien Thomas

    (Lausanne University Hospital, Geneva University Hospitals
    University of Lausanne)

  • Bernard L. Schneider

    (Brain Mind Institute, Ecole Polytechnique Fédérale de Lausanne (EPFL))

  • Philippe Renaud

    (Laboratory of Microsystems LMIS4, Ecole Polytechnique Fédérale de Lausanne (EPFL))

Abstract

Direct collection of extracellular fluid (ECF) plays a central role in the monitoring of neurological disorders. Current approaches using microdialysis catheters are however drastically limited in term of temporal resolution. Here we show a functional in vivo validation of a droplet collection system included at the tip of a neural probe. The system comprises an advanced droplet formation mechanism which enables the collection of neurochemicals present in the brain ECF at high-temporal resolution. The probe was implanted in a rat brain and could successfully collect fluid samples organized in a train of droplets. A microfabricated target plate compatible with most of the surface-based detection methods was specifically developed for sample analysis. The time-resolved brain-fluid samples are analyzed using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The results provide a time evolution picture of the cerebral tissues neurochemical composition for selected elements known for their involvement in neurodegenerative diseases.

Suggested Citation

  • Guillaume Petit-Pierre & Philippe Colin & Estelle Laurer & Julien Déglon & Arnaud Bertsch & Aurélien Thomas & Bernard L. Schneider & Philippe Renaud, 2017. "In vivo neurochemical measurements in cerebral tissues using a droplet-based monitoring system," Nature Communications, Nature, vol. 8(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01419-1
    DOI: 10.1038/s41467-017-01419-1
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