IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v8y2017i1d10.1038_s41467-017-01325-6.html
   My bibliography  Save this article

Live imaging molecular changes in junctional tension upon VE-cadherin in zebrafish

Author

Listed:
  • Anne Karine Lagendijk

    (Institute for Molecular Bioscience, Genomics of Development and Disease division, The University of Queensland)

  • Guillermo A. Gomez

    (The University of Queensland
    SA Pathology and the University of South Australia)

  • Sungmin Baek

    (Institute for Molecular Bioscience, Genomics of Development and Disease division, The University of Queensland)

  • Daniel Hesselson

    (Garvan Institute of Medical Research)

  • William E. Hughes

    (Garvan Institute of Medical Research)

  • Scott Paterson

    (Institute for Molecular Bioscience, Genomics of Development and Disease division, The University of Queensland)

  • Daniel E. Conway

    (Virginia Commonwealth University)

  • Heinz-Georg Belting

    (Biozentrum der Universität Basel)

  • Markus Affolter

    (Biozentrum der Universität Basel)

  • Kelly A. Smith

    (Institute for Molecular Bioscience, Genomics of Development and Disease division, The University of Queensland)

  • Martin A. Schwartz

    (Yale University School of Medicine)

  • Alpha S. Yap

    (The University of Queensland)

  • Benjamin M. Hogan

    (Institute for Molecular Bioscience, Genomics of Development and Disease division, The University of Queensland)

Abstract

Forces play diverse roles in vascular development, homeostasis and disease. VE-cadherin at endothelial cell-cell junctions links the contractile acto-myosin cytoskeletons of adjacent cells, serving as a tension-transducer. To explore tensile changes across VE-cadherin in live zebrafish, we tailored an optical biosensor approach, originally established in vitro. We validate localization and function of a VE-cadherin tension sensor (TS) in vivo. Changes in tension across VE-cadherin observed using ratio-metric or lifetime FRET measurements reflect acto-myosin contractility within endothelial cells. Furthermore, we apply the TS to reveal biologically relevant changes in VE-cadherin tension that occur as the dorsal aorta matures and upon genetic and chemical perturbations during embryonic development.

Suggested Citation

  • Anne Karine Lagendijk & Guillermo A. Gomez & Sungmin Baek & Daniel Hesselson & William E. Hughes & Scott Paterson & Daniel E. Conway & Heinz-Georg Belting & Markus Affolter & Kelly A. Smith & Martin A, 2017. "Live imaging molecular changes in junctional tension upon VE-cadherin in zebrafish," Nature Communications, Nature, vol. 8(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01325-6
    DOI: 10.1038/s41467-017-01325-6
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-017-01325-6
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-017-01325-6?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Jianmin Yin & Niels Schellinx & Ludovico Maggi & Kathrin Gundel & Cora Wiesner & Maria Paraskevi Kotini & Minkyoung Lee & Li-Kun Phng & Heinz-Georg Belting & Markus Affolter, 2024. "Initiation of lumen formation from junctions via differential actomyosin contractility regulated by dynamic recruitment of Rasip1," Nature Communications, Nature, vol. 15(1), pages 1-18, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01325-6. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.