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Structural basis for receptor recognition of pollen tube attraction peptides

Author

Listed:
  • Xiaoxiao Zhang

    (Tsinghua University)

  • Weijia Liu

    (Tsinghua University)

  • Takuya T. Nagae

    (Nagoya University)

  • Hidenori Takeuchi

    (Vienna Biocenter (VBC))

  • Heqiao Zhang

    (Tsinghua University)

  • Zhifu Han

    (Tsinghua University)

  • Tetsuya Higashiyama

    (Nagoya University
    Nagoya University
    Nagoya University)

  • Jijie Chai

    (Tsinghua University
    Max-Planck Institute for Plant Breeding Research
    University of Cologne)

Abstract

Transportation of the immobile sperms directed by pollen tubes to the ovule-enclosed female gametophytes is important for plant sexual reproduction. The defensin-like (DEFL) cysteine-rich peptides (CRPs) LUREs play an essential role in pollen tube attraction to the ovule, though their receptors still remain controversial. Here we provide several lines of biochemical evidence showing that the extracellular domain of the leucine-rich repeat receptor kinase (LRR-RK) PRK6 from Arabidopsis thaliana directly interacts with AtLURE1 peptides. Structural study reveals that a C-terminal loop of the LRR domain (AtPRK6LRR) is responsible for recognition of AtLURE1.2, mediated by a set of residues largely conserved among PRK6 homologs from Arabidopsis lyrata and Capsella rubella, supported by in vitro mutagenesis and semi-in-vivo pollen tube growth assays. Our study provides evidence showing that PRK6 functions as a receptor of the LURE peptides in A. thaliana and reveals a unique ligand recognition mechanism of LRR-RKs.

Suggested Citation

  • Xiaoxiao Zhang & Weijia Liu & Takuya T. Nagae & Hidenori Takeuchi & Heqiao Zhang & Zhifu Han & Tetsuya Higashiyama & Jijie Chai, 2017. "Structural basis for receptor recognition of pollen tube attraction peptides," Nature Communications, Nature, vol. 8(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01323-8
    DOI: 10.1038/s41467-017-01323-8
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    Cited by:

    1. Yuebin Wang & Wenqiang Li & Luxi Wang & Jiali Yan & Gang Lu & Ning Yang & Jieting Xu & Yuqing Wang & Songtao Gui & Gengshen Chen & Shuyan Li & Chengxiu Wu & Tingting Guo & Yingjie Xiao & Marilyn L. Wa, 2022. "Three types of genes underlying the Gametophyte factor1 locus cause unilateral cross incompatibility in maize," Nature Communications, Nature, vol. 13(1), pages 1-12, December.

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