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Achieving high-efficiency emission depletion nanoscopy by employing cross relaxation in upconversion nanoparticles

Author

Listed:
  • Qiuqiang Zhan

    (South China Academy of Advanced Optoelectronics, South China Normal University)

  • Haichun Liu

    (Royal Institute of Technology)

  • Baoju Wang

    (South China Academy of Advanced Optoelectronics, South China Normal University)

  • Qiusheng Wu

    (South China Academy of Advanced Optoelectronics, South China Normal University)

  • Rui Pu

    (South China Academy of Advanced Optoelectronics, South China Normal University)

  • Chao Zhou

    (South China Academy of Advanced Optoelectronics, South China Normal University)

  • Bingru Huang

    (South China Academy of Advanced Optoelectronics, South China Normal University)

  • Xingyun Peng

    (South China Academy of Advanced Optoelectronics, South China Normal University)

  • Hans Ågren

    (Royal Institute of Technology)

  • Sailing He

    (South China Academy of Advanced Optoelectronics, South China Normal University
    State Key Laboratory of Modern Optical Instrumentation, Zhejiang University
    Royal Institute of Technology)

Abstract

Stimulated emission depletion microscopy provides a powerful sub-diffraction imaging modality for life science studies. Conventionally, stimulated emission depletion requires a relatively high light intensity to obtain an adequate depletion efficiency through only light–matter interaction. Here we show efficient emission depletion for a class of lanthanide-doped upconversion nanoparticles with the assistance of interionic cross relaxation, which significantly lowers the laser intensity requirements of optical depletion. We demonstrate two-color super-resolution imaging using upconversion nanoparticles (resolution ~ 66 nm) with a single pair of excitation/depletion beams. In addition, we show super-resolution imaging of immunostained cytoskeleton structures of fixed cells (resolution ~ 82 nm) using upconversion nanoparticles. These achievements provide a new perspective for the development of photoswitchable luminescent probes and will broaden the applications of lanthanide-doped nanoparticles for sub-diffraction microscopic imaging.

Suggested Citation

  • Qiuqiang Zhan & Haichun Liu & Baoju Wang & Qiusheng Wu & Rui Pu & Chao Zhou & Bingru Huang & Xingyun Peng & Hans Ågren & Sailing He, 2017. "Achieving high-efficiency emission depletion nanoscopy by employing cross relaxation in upconversion nanoparticles," Nature Communications, Nature, vol. 8(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01141-y
    DOI: 10.1038/s41467-017-01141-y
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    Cited by:

    1. Rui Pu & Qiuqiang Zhan & Xingyun Peng & Siying Liu & Xin Guo & Liangliang Liang & Xian Qin & Ziqing Winston Zhao & Xiaogang Liu, 2022. "Super-resolution microscopy enabled by high-efficiency surface-migration emission depletion," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    2. Xiumei Yin & Wen Xu & Ge Zhu & Yanan Ji & Qi Xiao & Xinyao Dong & Ming He & Baosheng Cao & Na Zhou & Xixian Luo & Lin Guo & Bin Dong, 2022. "Towards highly efficient NIR II response up-conversion phosphor enabled by long lifetimes of Er3+," Nature Communications, Nature, vol. 13(1), pages 1-10, December.

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