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Calmodulin dissociates the STIM1-Orai1 complex and STIM1 oligomers

Author

Listed:
  • Xin Li

    (Nankai University)

  • Guangyan Wu

    (Nankai University)

  • Yin Yang

    (Nankai University)

  • Shijuan Fu

    (Nankai University)

  • Xiaofen Liu

    (Nankai University)

  • Huimin Kang

    (Nankai University)

  • Xue Yang

    (Nankai University)

  • Xun-Cheng Su

    (Nankai University
    Synergetic Innovation Center of Chemical Science and Engineering)

  • Yuequan Shen

    (Nankai University
    Synergetic Innovation Center of Chemical Science and Engineering)

Abstract

Store-operated calcium entry (SOCE) is a major pathway for calcium ions influx into cells and has a critical role in various cell functions. Here we demonstrate that calcium-bound calmodulin (Ca2+-CaM) binds to the core region of activated STIM1. This interaction facilitates slow Ca2+-dependent inactivation after Orai1 channel activation by wild-type STIM1 or a constitutively active STIM1 mutant. We define the CaM-binding site in STIM1, which is adjacent to the STIM1–Orai1 coupling region. The binding of Ca2+-CaM to activated STIM1 disrupts the STIM1–Orai1 complex and also disassembles STIM1 oligomer. Based on these results we propose a model for the calcium-bound CaM-regulated deactivation of SOCE.

Suggested Citation

  • Xin Li & Guangyan Wu & Yin Yang & Shijuan Fu & Xiaofen Liu & Huimin Kang & Xue Yang & Xun-Cheng Su & Yuequan Shen, 2017. "Calmodulin dissociates the STIM1-Orai1 complex and STIM1 oligomers," Nature Communications, Nature, vol. 8(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01135-w
    DOI: 10.1038/s41467-017-01135-w
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    Cited by:

    1. Kihyoun Park & Hyejin Lim & Jinyoung Kim & Yeseong Hwang & Yu Seol Lee & Soo Han Bae & Hyeongseok Kim & Hail Kim & Shin-Wook Kang & Joo Young Kim & Myung-Shik Lee, 2022. "Lysosomal Ca2+-mediated TFEB activation modulates mitophagy and functional adaptation of pancreatic β-cells to metabolic stress," Nature Communications, Nature, vol. 13(1), pages 1-17, December.

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