IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v8y2017i1d10.1038_s41467-017-01075-5.html
   My bibliography  Save this article

A structural model of flagellar filament switching across multiple bacterial species

Author

Listed:
  • Fengbin Wang

    (University of Virginia School of Medicine)

  • Andrew M. Burrage

    (Indiana University)

  • Sandra Postel

    (Institute of Human Virology and University of Maryland School of Medicine)

  • Reece E. Clark

    (Indiana University)

  • Albina Orlova

    (University of Virginia School of Medicine)

  • Eric J. Sundberg

    (Institute of Human Virology and University of Maryland School of Medicine
    University of Maryland School of Medicine)

  • Daniel B. Kearns

    (Indiana University)

  • Edward H. Egelman

    (University of Virginia School of Medicine)

Abstract

The bacterial flagellar filament has long been studied to understand how a polymer composed of a single protein can switch between different supercoiled states with high cooperativity. Here we present near-atomic resolution cryo-EM structures for flagellar filaments from both Gram-positive Bacillus subtilis and Gram-negative Pseudomonas aeruginosa. Seven mutant flagellar filaments in B. subtilis and two in P. aeruginosa capture two different states of the filament. These reliable atomic models of both states reveal conserved molecular interactions in the interior of the filament among B. subtilis, P. aeruginosa and Salmonella enterica. Using the detailed information about the molecular interactions in two filament states, we successfully predict point mutations that shift the equilibrium between those two states. Further, we observe the dimerization of P. aeruginosa outer domains without any perturbation of the conserved interior of the filament. Our results give new insights into how the flagellin sequence has been “tuned” over evolution.

Suggested Citation

  • Fengbin Wang & Andrew M. Burrage & Sandra Postel & Reece E. Clark & Albina Orlova & Eric J. Sundberg & Daniel B. Kearns & Edward H. Egelman, 2017. "A structural model of flagellar filament switching across multiple bacterial species," Nature Communications, Nature, vol. 8(1), pages 1-13, December.
  • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01075-5
    DOI: 10.1038/s41467-017-01075-5
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-017-01075-5
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/s41467-017-01075-5?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Nicholas M Thomson & Josie L Ferreira & Teige R Matthews-Palmer & Morgan Beeby & Mark J Pallen, 2018. "Giant flagellins form thick flagellar filaments in two species of marine γ-proteobacteria," PLOS ONE, Public Library of Science, vol. 13(11), pages 1-12, November.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01075-5. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.