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Three-dimensional scanless holographic optogenetics with temporal focusing (3D-SHOT)

Author

Listed:
  • Nicolas C. Pégard

    (University of California
    University of California)

  • Alan R. Mardinly

    (University of California)

  • Ian Antón Oldenburg

    (University of California)

  • Savitha Sridharan

    (University of California)

  • Laura Waller

    (University of California)

  • Hillel Adesnik

    (University of California
    University of California)

Abstract

Optical methods capable of manipulating neural activity with cellular resolution and millisecond precision in three dimensions will accelerate the pace of neuroscience research. Existing approaches for targeting individual neurons, however, fall short of these requirements. Here we present a new multiphoton photo-excitation method, termed three-dimensional scanless holographic optogenetics with temporal focusing (3D-SHOT), which allows precise, simultaneous photo-activation of arbitrary sets of neurons anywhere within the addressable volume of a microscope. This technique uses point-cloud holography to place multiple copies of a temporally focused disc matching the dimensions of a neuron’s cell body. Experiments in cultured cells, brain slices, and in living mice demonstrate single-neuron spatial resolution even when optically targeting randomly distributed groups of neurons in 3D. This approach opens new avenues for mapping and manipulating neural circuits, allowing a real-time, cellular resolution interface to the brain.

Suggested Citation

  • Nicolas C. Pégard & Alan R. Mardinly & Ian Antón Oldenburg & Savitha Sridharan & Laura Waller & Hillel Adesnik, 2017. "Three-dimensional scanless holographic optogenetics with temporal focusing (3D-SHOT)," Nature Communications, Nature, vol. 8(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01031-3
    DOI: 10.1038/s41467-017-01031-3
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    Cited by:

    1. Carlos A. Z. Bassetto & Juergen Pfeffermann & Rohit Yadav & Simon Strassgschwandtner & Toma Glasnov & Francisco Bezanilla & Peter Pohl, 2024. "Photolipid excitation triggers depolarizing optocapacitive currents and action potentials," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

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