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Single-molecule study of oxidative enzymatic deconstruction of cellulose

Author

Listed:
  • Manuel Eibinger

    (Graz University of Technology)

  • Jürgen Sattelkow

    (Graz University of Technology)

  • Thomas Ganner

    (Graz University of Technology)

  • Harald Plank

    (Graz University of Technology
    Graz Centre for Electron Microscopy)

  • Bernd Nidetzky

    (Graz University of Technology
    Austrian Centre of Industrial Biotechnology)

Abstract

LPMO (lytic polysaccharide monooxygenase) represents a unique paradigm of cellulosic biomass degradation by an oxidative mechanism. Understanding the role of LPMO in deconstructing crystalline cellulose is fundamental to the enzyme’s biological function and will help to specify the use of LPMO in biorefinery applications. Here we show with real-time atomic force microscopy that C1 and C4 oxidizing types of LPMO from Neurospora crassa (NcLPMO9F, NcLPMO9C) bind to nanocrystalline cellulose with high preference for the very same substrate surfaces that are also used by a processive cellulase (Trichoderma reesei CBH I) to move along during hydrolytic cellulose degradation. The bound LPMOs, however, are immobile during their adsorbed residence time ( ~ 1.0 min for NcLPMO9F) on cellulose. Treatment with LPMO resulted in fibrillation of crystalline cellulose and strongly ( ≥ 2-fold) enhanced the cellulase adsorption. It also increased enzyme turnover on the cellulose surface, thus boosting the hydrolytic conversion.

Suggested Citation

  • Manuel Eibinger & Jürgen Sattelkow & Thomas Ganner & Harald Plank & Bernd Nidetzky, 2017. "Single-molecule study of oxidative enzymatic deconstruction of cellulose," Nature Communications, Nature, vol. 8(1), pages 1-7, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01028-y
    DOI: 10.1038/s41467-017-01028-y
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    Cited by:

    1. Wen-Xin Jiang & Ping-Yi Li & Xiu-Lan Chen & Yi-Shuo Zhang & Jing-Ping Wang & Yan-Jun Wang & Qi Sheng & Zhong-Zhi Sun & Qi-Long Qin & Xue-Bing Ren & Peng Wang & Xiao-Yan Song & Yin Chen & Yu-Zhong Zhan, 2022. "A pathway for chitin oxidation in marine bacteria," Nature Communications, Nature, vol. 13(1), pages 1-15, December.

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