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Structural insights into the competitive inhibition of the ATP-gated P2X receptor channel

Author

Listed:
  • Go Kasuya

    (The University of Tokyo)

  • Toshiaki Yamaura

    (Asahi Kasei Pharma Corporation)

  • Xiao-Bo Ma

    (Institute of Medical Sciences, School of Medicine, Shanghai Jiao Tong University)

  • Ryoki Nakamura

    (The University of Tokyo)

  • Mizuki Takemoto

    (The University of Tokyo)

  • Hiromitsu Nagumo

    (Asahi Kasei Pharma Corporation)

  • Eiichi Tanaka

    (Asahi Kasei Pharma Corporation)

  • Naoshi Dohmae

    (Global Research Cluster, RIKEN)

  • Takanori Nakane

    (The University of Tokyo)

  • Ye Yu

    (Institute of Medical Sciences, School of Medicine, Shanghai Jiao Tong University)

  • Ryuichiro Ishitani

    (The University of Tokyo)

  • Osamu Matsuzaki

    (Asahi Kasei Pharma Corporation)

  • Motoyuki Hattori

    (Fudan University)

  • Osamu Nureki

    (The University of Tokyo)

Abstract

P2X receptors are non-selective cation channels gated by extracellular ATP, and the P2X7 receptor subtype plays a crucial role in the immune and nervous systems. Altered expression and dysfunctions of P2X7 receptors caused by genetic deletions, mutations, and polymorphic variations have been linked to various diseases, such as rheumatoid arthritis and hypertension. Despite the availability of crystal structures of P2X receptors, the mechanism of competitive antagonist action for P2X receptors remains controversial. Here, we determine the crystal structure of the chicken P2X7 receptor in complex with the competitive P2X antagonist, TNP-ATP. The structure reveals an expanded, incompletely activated conformation of the channel, and identified the unique recognition manner of TNP-ATP, which is distinct from that observed in the previously determined human P2X3 receptor structure. A structure-based computational analysis furnishes mechanistic insights into the TNP-ATP-dependent inhibition. Our work provides structural insights into the functional mechanism of the P2X competitive antagonist.

Suggested Citation

  • Go Kasuya & Toshiaki Yamaura & Xiao-Bo Ma & Ryoki Nakamura & Mizuki Takemoto & Hiromitsu Nagumo & Eiichi Tanaka & Naoshi Dohmae & Takanori Nakane & Ye Yu & Ryuichiro Ishitani & Osamu Matsuzaki & Motoy, 2017. "Structural insights into the competitive inhibition of the ATP-gated P2X receptor channel," Nature Communications, Nature, vol. 8(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00887-9
    DOI: 10.1038/s41467-017-00887-9
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    Cited by:

    1. Adam C. Oken & Nicolas E. Lisi & Ipsita Krishnamurthy & Alanna E. McCarthy & Michael H. Godsey & Arthur Glasfeld & Steven E. Mansoor, 2024. "High-affinity agonism at the P2X7 receptor is mediated by three residues outside the orthosteric pocket," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    2. Cheng Shen & Yuqing Zhang & Wenwen Cui & Yimeng Zhao & Danqi Sheng & Xinyu Teng & Miaoqing Shao & Muneyoshi Ichikawa & Jin Wang & Motoyuki Hattori, 2023. "Structural insights into the allosteric inhibition of P2X4 receptors," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    3. Felix M. Bennetts & Hariprasad Venugopal & Alisa Glukhova & Jesse I. Mobbs & Sabatino Ventura & David M. Thal, 2024. "Structural insights into the human P2X1 receptor and ligand interactions," Nature Communications, Nature, vol. 15(1), pages 1-14, December.

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