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A general mechanism of ribosome dimerization revealed by single-particle cryo-electron microscopy

Author

Listed:
  • Linda E. Franken

    (University of Groningen)

  • Gert T. Oostergetel

    (University of Groningen)

  • Tjaard Pijning

    (University of Groningen)

  • Pranav Puri

    (University of Groningen)

  • Valentina Arkhipova

    (University of Groningen)

  • Egbert J. Boekema

    (University of Groningen)

  • Bert Poolman

    (University of Groningen)

  • Albert Guskov

    (University of Groningen)

Abstract

Bacteria downregulate their ribosomal activity through dimerization of 70S ribosomes, yielding inactive 100S complexes. In Escherichia coli, dimerization is mediated by the hibernation promotion factor (HPF) and ribosome modulation factor. Here we report the cryo-electron microscopy study on 100S ribosomes from Lactococcus lactis and a dimerization mechanism involving a single protein: HPFlong. The N-terminal domain of HPFlong binds at the same site as HPF in Escherichia coli 100S ribosomes. Contrary to ribosome modulation factor, the C-terminal domain of HPFlong binds exactly at the dimer interface. Furthermore, ribosomes from Lactococcus lactis do not undergo conformational changes in the 30S head domains upon binding of HPFlong, and the Shine–Dalgarno sequence and mRNA entrance tunnel remain accessible. Ribosome activity is blocked by HPFlong due to the inhibition of mRNA recognition by the platform binding center. Phylogenetic analysis of HPF proteins suggests that HPFlong-mediated dimerization is a widespread mechanism of ribosome hibernation in bacteria.

Suggested Citation

  • Linda E. Franken & Gert T. Oostergetel & Tjaard Pijning & Pranav Puri & Valentina Arkhipova & Egbert J. Boekema & Bert Poolman & Albert Guskov, 2017. "A general mechanism of ribosome dimerization revealed by single-particle cryo-electron microscopy," Nature Communications, Nature, vol. 8(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00718-x
    DOI: 10.1038/s41467-017-00718-x
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    Cited by:

    1. Niraj Kumar & Shivani Sharma & Prem S. Kaushal, 2024. "Cryo- EM structure of the mycobacterial 70S ribosome in complex with ribosome hibernation promotion factor RafH," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

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