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RNA localization is a key determinant of neurite-enriched proteome

Author

Listed:
  • Alessandra Zappulo

    (Max Delbrück Center for Molecular Medicine)

  • David Bruck

    (Max Delbrück Center for Molecular Medicine)

  • Camilla Ciolli Mattioli

    (Max Delbrück Center for Molecular Medicine)

  • Vedran Franke

    (Max Delbrück Center for Molecular Medicine)

  • Koshi Imami

    (Max Delbrück Center for Molecular Medicine)

  • Erik McShane

    (Max Delbrück Center for Molecular Medicine)

  • Mireia Moreno-Estelles

    (New York University)

  • Lorenzo Calviello

    (Max Delbrück Center for Molecular Medicine)

  • Andrei Filipchyk

    (Max Delbrück Center for Molecular Medicine)

  • Esteban Peguero-Sanchez

    (Max Delbrück Center for Molecular Medicine
    UNAM, Av. Universidad 2001)

  • Thomas Müller

    (Max Delbrück Center for Molecular Medicine)

  • Andrew Woehler

    (Max Delbrück Center for Molecular Medicine)

  • Carmen Birchmeier

    (Max Delbrück Center for Molecular Medicine)

  • Enrique Merino

    (UNAM, Av. Universidad 2001)

  • Nikolaus Rajewsky

    (Max Delbrück Center for Molecular Medicine)

  • Uwe Ohler

    (Max Delbrück Center for Molecular Medicine)

  • Esteban O. Mazzoni

    (New York University)

  • Matthias Selbach

    (Max Delbrück Center for Molecular Medicine)

  • Altuna Akalin

    (Max Delbrück Center for Molecular Medicine)

  • Marina Chekulaeva

    (Max Delbrück Center for Molecular Medicine)

Abstract

Protein subcellular localization is fundamental to the establishment of the body axis, cell migration, synaptic plasticity, and a vast range of other biological processes. Protein localization occurs through three mechanisms: protein transport, mRNA localization, and local translation. However, the relative contribution of each process to neuronal polarity remains unknown. Using neurons differentiated from mouse embryonic stem cells, we analyze protein and RNA expression and translation rates in isolated cell bodies and neurites genome-wide. We quantify 7323 proteins and the entire transcriptome, and identify hundreds of neurite-localized proteins and locally translated mRNAs. Our results demonstrate that mRNA localization is the primary mechanism for protein localization in neurites that may account for half of the neurite-localized proteome. Moreover, we identify multiple neurite-targeted non-coding RNAs and RNA-binding proteins with potential regulatory roles. These results provide further insight into the mechanisms underlying the establishment of neuronal polarity.

Suggested Citation

  • Alessandra Zappulo & David Bruck & Camilla Ciolli Mattioli & Vedran Franke & Koshi Imami & Erik McShane & Mireia Moreno-Estelles & Lorenzo Calviello & Andrei Filipchyk & Esteban Peguero-Sanchez & Thom, 2017. "RNA localization is a key determinant of neurite-enriched proteome," Nature Communications, Nature, vol. 8(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00690-6
    DOI: 10.1038/s41467-017-00690-6
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    Cited by:

    1. Haofan Sun & Bin Fu & Xiaohong Qian & Ping Xu & Weijie Qin, 2024. "Nuclear and cytoplasmic specific RNA binding proteome enrichment and its changes upon ferroptosis induction," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

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