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Landscape of submitochondrial protein distribution

Author

Listed:
  • F.-Nora Vögtle

    (University of Freiburg)

  • Julia M. Burkhart

    (Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V)

  • Humberto Gonczarowska-Jorge

    (Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V)

  • Cansu Kücükköse

    (University of Freiburg
    University of Freiburg)

  • Asli Aras Taskin

    (University of Freiburg)

  • Dominik Kopczynski

    (Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V)

  • Robert Ahrends

    (Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V)

  • Dirk Mossmann

    (University of Freiburg)

  • Albert Sickmann

    (Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V
    University of Aberdeen
    Ruhr Universität Bochum)

  • René P. Zahedi

    (Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V)

  • Chris Meisinger

    (University of Freiburg
    University of Freiburg)

Abstract

The mitochondrial proteome comprises ~1000 (yeast)–1500 (human) different proteins, which are distributed into four different subcompartments. The sublocalization of these proteins within the organelle in most cases remains poorly defined. Here we describe an integrated approach combining stable isotope labeling, various protein enrichment and extraction strategies and quantitative mass spectrometry to produce a quantitative map of submitochondrial protein distribution in S. cerevisiae. This quantitative landscape enables a proteome-wide classification of 986 proteins into soluble, peripheral, and integral mitochondrial membrane proteins, and the assignment of 818 proteins into the four subcompartments: outer membrane, inner membrane, intermembrane space, or matrix. We also identified 206 proteins that were not previously annotated as localized to mitochondria. Furthermore, the protease Prd1, misannotated as intermembrane space protein, could be re-assigned and characterized as a presequence peptide degrading enzyme in the matrix.

Suggested Citation

  • F.-Nora Vögtle & Julia M. Burkhart & Humberto Gonczarowska-Jorge & Cansu Kücükköse & Asli Aras Taskin & Dominik Kopczynski & Robert Ahrends & Dirk Mossmann & Albert Sickmann & René P. Zahedi & Chris M, 2017. "Landscape of submitochondrial protein distribution," Nature Communications, Nature, vol. 8(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00359-0
    DOI: 10.1038/s41467-017-00359-0
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    Cited by:

    1. Ying Zhu & Kerem Can Akkaya & Julia Ruta & Nanako Yokoyama & Cong Wang & Max Ruwolt & Diogo Borges Lima & Martin Lehmann & Fan Liu, 2024. "Cross-link assisted spatial proteomics to map sub-organelle proteomes and membrane protein topologies," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    2. Shiqun Wang & Wei Yan & Lingkai Kong & Shuguang Zuo & Jingyi Wu & Chunxiao Zhu & Huaping Huang & Bohao He & Jie Dong & Jiwu Wei, 2023. "Oncolytic viruses engineered to enforce cholesterol efflux restore tumor-associated macrophage phagocytosis and anti-tumor immunity in glioblastoma," Nature Communications, Nature, vol. 14(1), pages 1-21, December.

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