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Ensa controls S-phase length by modulating Treslin levels

Author

Listed:
  • Sophie Charrasse

    (Université de Montpellier, Centre de Recherche de Biologie Cellulaire de Montpellier, Equipe Labellisée ‘Ligue Contre le Cancer’, CNRS UMR 5237)

  • Aicha Gharbi-Ayachi

    (Université de Montpellier, Centre de Recherche de Biologie Cellulaire de Montpellier, Equipe Labellisée ‘Ligue Contre le Cancer’, CNRS UMR 5237)

  • Andrew Burgess

    (Garvan Institute of Medical Research
    UNSW)

  • Jorge Vera

    (Université de Montpellier, Centre de Recherche de Biologie Cellulaire de Montpellier, Equipe Labellisée ‘Ligue Contre le Cancer’, CNRS UMR 5237)

  • Khaled Hached

    (Université de Montpellier, Centre de Recherche de Biologie Cellulaire de Montpellier, Equipe Labellisée ‘Ligue Contre le Cancer’, CNRS UMR 5237)

  • Peggy Raynaud

    (Université de Montpellier, Centre de Recherche de Biologie Cellulaire de Montpellier, Equipe Labellisée ‘Ligue Contre le Cancer’, CNRS UMR 5237)

  • Etienne Schwob

    (CNRS UMR 5535, University of Montpellier)

  • Thierry Lorca

    (Université de Montpellier, Centre de Recherche de Biologie Cellulaire de Montpellier, Equipe Labellisée ‘Ligue Contre le Cancer’, CNRS UMR 5237)

  • Anna Castro

    (Université de Montpellier, Centre de Recherche de Biologie Cellulaire de Montpellier, Equipe Labellisée ‘Ligue Contre le Cancer’, CNRS UMR 5237)

Abstract

The Greatwall/Ensa/PP2A-B55 pathway is essential for controlling mitotic substrate phosphorylation and mitotic entry. Here, we investigate the effect of the knockdown of the Gwl substrate, Ensa, in human cells. Unexpectedly, Ensa knockdown promotes a dramatic extension of S phase associated with a lowered density of replication forks. Notably, Ensa depletion results in a decrease of Treslin levels, a pivotal protein for the firing of replication origins. Accordingly, the extended S phase in Ensa-depleted cells is completely rescued by the overexpression of Treslin. Our data herein reveal a new mechanism by which normal cells regulate S-phase duration by controlling the ubiquitin-proteasome degradation of Treslin in a Gwl/Ensa-dependent pathway.

Suggested Citation

  • Sophie Charrasse & Aicha Gharbi-Ayachi & Andrew Burgess & Jorge Vera & Khaled Hached & Peggy Raynaud & Etienne Schwob & Thierry Lorca & Anna Castro, 2017. "Ensa controls S-phase length by modulating Treslin levels," Nature Communications, Nature, vol. 8(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00339-4
    DOI: 10.1038/s41467-017-00339-4
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    Cited by:

    1. Yi-Yu Chen & Jing-Yu Ge & Si-Yuan Zhu & Zhi-Ming Shao & Ke-Da Yu, 2022. "Copy number amplification of ENSA promotes the progression of triple-negative breast cancer via cholesterol biosynthesis," Nature Communications, Nature, vol. 13(1), pages 1-16, December.

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