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Aurora-B kinase pathway controls the lateral to end-on conversion of kinetochore-microtubule attachments in human cells

Author

Listed:
  • Roshan L. Shrestha

    (University of Cambridge
    National Institutes of Health)

  • Duccio Conti

    (University of Cambridge
    Queen Mary University of London)

  • Naoka Tamura

    (University of Cambridge
    Queen Mary University of London)

  • Dominique Braun

    (University of Cambridge)

  • Revathy A. Ramalingam

    (Queen Mary University of London)

  • Konstanty Cieslinski

    (Cell Biology and Biophysics Unit)

  • Jonas Ries

    (Cell Biology and Biophysics Unit)

  • Viji M. Draviam

    (University of Cambridge
    Queen Mary University of London)

Abstract

Human chromosomes are captured along microtubule walls (lateral attachment) and then tethered to microtubule-ends (end-on attachment) through a multi-step end-on conversion process. Upstream regulators that orchestrate this remarkable change in the plane of kinetochore-microtubule attachment in human cells are not known. By tracking kinetochore movements and using kinetochore markers specific to attachment status, we reveal a spatially defined role for Aurora-B kinase in retarding the end-on conversion process. To understand how Aurora-B activity is counteracted, we compare the roles of two outer-kinetochore bound phosphatases and find that BubR1-associated PP2A, unlike KNL1-associated PP1, plays a significant role in end-on conversion. Finally, we uncover a novel role for Aurora-B regulated Astrin-SKAP complex in ensuring the correct plane of kinetochore-microtubule attachment. Thus, we identify Aurora-B as a key upstream regulator of end-on conversion in human cells and establish a late role for Astrin-SKAP complex in the end-on conversion process.

Suggested Citation

  • Roshan L. Shrestha & Duccio Conti & Naoka Tamura & Dominique Braun & Revathy A. Ramalingam & Konstanty Cieslinski & Jonas Ries & Viji M. Draviam, 2017. "Aurora-B kinase pathway controls the lateral to end-on conversion of kinetochore-microtubule attachments in human cells," Nature Communications, Nature, vol. 8(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00209-z
    DOI: 10.1038/s41467-017-00209-z
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    Cited by:

    1. Seungyeul Yoo & Abhilasha Sinha & Dawei Yang & Nasser K. Altorki & Radhika Tandon & Wenhui Wang & Deebly Chavez & Eunjee Lee & Ayushi S. Patel & Takashi Sato & Ranran Kong & Bisen Ding & Eric E. Schad, 2022. "Integrative network analysis of early-stage lung adenocarcinoma identifies aurora kinase inhibition as interceptor of invasion and progression," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    2. Jurica Matković & Subhadip Ghosh & Mateja Ćosić & Susana Eibes & Marin Barišić & Nenad Pavin & Iva M. Tolić, 2022. "Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assembly," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    3. Shuzhen Yang & Mengya Cai & Junjie Huang & Shengnan Zhang & Xiaoli Mo & Kai Jiang & Huiting Cui & Jing Yuan, 2023. "EB1 decoration of microtubule lattice facilitates spindle-kinetochore lateral attachment in Plasmodium male gametogenesis," Nature Communications, Nature, vol. 14(1), pages 1-20, December.

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