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Negri bodies are viral factories with properties of liquid organelles

Author

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  • Jovan Nikolic

    (Univ. Paris-Sud, Université Paris-Saclay)

  • Romain Le Bars

    (Univ. Paris-Sud, Université Paris-Saclay)

  • Zoé Lama

    (Univ. Paris-Sud, Université Paris-Saclay)

  • Nathalie Scrima

    (Univ. Paris-Sud, Université Paris-Saclay)

  • Cécile Lagaudrière-Gesbert

    (Univ. Paris-Sud, Université Paris-Saclay)

  • Yves Gaudin

    (Univ. Paris-Sud, Université Paris-Saclay)

  • Danielle Blondel

    (Univ. Paris-Sud, Université Paris-Saclay)

Abstract

Replication of Mononegavirales occurs in viral factories which form inclusions in the host-cell cytoplasm. For rabies virus, those inclusions are called Negri bodies (NBs). We report that NBs have characteristics similar to those of liquid organelles: they are spherical, they fuse to form larger structures, and they disappear upon hypotonic shock. Their liquid phase is confirmed by FRAP experiments. Live-cell imaging indicates that viral nucleocapsids are ejected from NBs and transported along microtubules to form either new virions or secondary viral factories. Coexpression of rabies virus N and P proteins results in cytoplasmic inclusions recapitulating NBs properties. This minimal system reveals that an intrinsically disordered domain and the dimerization domain of P are essential for Negri bodies-like structures formation. We suggest that formation of liquid viral factories by phase separation is common among Mononegavirales and allows specific recruitment and concentration of viral proteins but also the escape to cellular antiviral response.

Suggested Citation

  • Jovan Nikolic & Romain Le Bars & Zoé Lama & Nathalie Scrima & Cécile Lagaudrière-Gesbert & Yves Gaudin & Danielle Blondel, 2017. "Negri bodies are viral factories with properties of liquid organelles," Nature Communications, Nature, vol. 8(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00102-9
    DOI: 10.1038/s41467-017-00102-9
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    Cited by:

    1. Jingru Fang & Guillaume Castillon & Sebastien Phan & Sara McArdle & Chitra Hariharan & Aiyana Adams & Mark H. Ellisman & Ashok A. Deniz & Erica Ollmann Saphire, 2023. "Spatial and functional arrangement of Ebola virus polymerase inside phase-separated viral factories," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    2. Jiang Ren & Shuai Wang & Zhi Zong & Ting Pan & Sijia Liu & Wei Mao & Huizhe Huang & Xiaohua Yan & Bing Yang & Xin He & Fangfang Zhou & Long Zhang, 2024. "TRIM28-mediated nucleocapsid protein SUMOylation enhances SARS-CoV-2 virulence," Nature Communications, Nature, vol. 15(1), pages 1-19, December.

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