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ATP-dependent modulation of MgtE in Mg2+ homeostasis

Author

Listed:
  • Atsuhiro Tomita

    (The University of Tokyo)

  • Mingfeng Zhang

    (Fudan University)

  • Fei Jin

    (Fudan University)

  • Wenhui Zhuang

    (Fudan University)

  • Hironori Takeda

    (Kyoto Sangyo University, Kamigamo-motoyama)

  • Tatsuro Maruyama

    (The University of Tokyo, Hongo)

  • Masanori Osawa

    (The University of Tokyo, Hongo)

  • Ken-ichi Hashimoto

    (Tokyo Denki University)

  • Hisashi Kawasaki

    (Tokyo Denki University)

  • Koichi Ito

    (The University of Tokyo)

  • Naoshi Dohmae

    (RIKEN Center for Sustainable Resource Science)

  • Ryuichiro Ishitani

    (The University of Tokyo)

  • Ichio Shimada

    (The University of Tokyo, Hongo)

  • Zhiqiang Yan

    (Fudan University
    Southwest Medical University)

  • Motoyuki Hattori

    (Fudan University)

  • Osamu Nureki

    (The University of Tokyo)

Abstract

Magnesium is an essential ion for numerous physiological processes. MgtE is a Mg2+ selective channel involved in the maintenance of intracellular Mg2+ homeostasis, whose gating is regulated by intracellular Mg2+ levels. Here, we report that ATP binds to MgtE, regulating its Mg2+-dependent gating. Crystal structures of MgtE–ATP complex show that ATP binds to the intracellular CBS domain of MgtE. Functional studies support that ATP binding to MgtE enhances the intracellular domain affinity for Mg2+ within physiological concentrations of this divalent cation, enabling MgtE to function as an in vivo Mg2+ sensor. ATP dissociation from MgtE upregulates Mg2+ influx at both high and low intracellular Mg2+ concentrations. Using site-directed mutagenesis and structure based-electrophysiological and biochemical analyses, we identify key residues and main structural changes involved in the process. This work provides the molecular basis of ATP-dependent modulation of MgtE in Mg2+ homeostasis.

Suggested Citation

  • Atsuhiro Tomita & Mingfeng Zhang & Fei Jin & Wenhui Zhuang & Hironori Takeda & Tatsuro Maruyama & Masanori Osawa & Ken-ichi Hashimoto & Hisashi Kawasaki & Koichi Ito & Naoshi Dohmae & Ryuichiro Ishita, 2017. "ATP-dependent modulation of MgtE in Mg2+ homeostasis," Nature Communications, Nature, vol. 8(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00082-w
    DOI: 10.1038/s41467-017-00082-w
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    Cited by:

    1. Louis Tung Faat Lai & Jayashree Balaraman & Fei Zhou & Doreen Matthies, 2023. "Cryo-EM structures of human magnesium channel MRS2 reveal gating and regulatory mechanisms," Nature Communications, Nature, vol. 14(1), pages 1-10, December.

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