IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v8y2017i1d10.1038_s41467-017-00053-1.html
   My bibliography  Save this article

PfCDPK1 mediated signaling in erythrocytic stages of Plasmodium falciparum

Author

Listed:
  • Sudhir Kumar

    (National Institute of Immunology)

  • Manish Kumar

    (Institute of Bioinformatics
    Manipal University)

  • Roseleen Ekka

    (National Institute of Immunology)

  • Jeffrey D. Dvorin

    (Harvard University
    Boston Children’s Hospital and Harvard Medical School)

  • Aditya S. Paul

    (Harvard University)

  • Anil K. Madugundu

    (Institute of Bioinformatics
    Pondicherry University)

  • Tim Gilberger

    (Bernhard Nocht Institute for Tropical Medicine and Centre for Structural Systems Biology)

  • Harsha Gowda

    (Institute of Bioinformatics
    Yenepoya University)

  • Manoj T. Duraisingh

    (Harvard University)

  • T. S. Keshava Prasad

    (Institute of Bioinformatics
    Yenepoya University
    National Institute of Mental Health and Neuro Sciences)

  • Pushkar Sharma

    (National Institute of Immunology)

Abstract

Calcium Dependent Protein Kinases are key effectors of calcium signaling in malaria parasite. PfCDPK1 is critical for asexual development of Plasmodium falciparum, but its precise function and substrates remain largely unknown. Using a conditional knockdown strategy, we here establish that this kinase is critical for the invasion of host erythrocytes. Furthermore, using a multidisciplinary approach involving comparative phosphoproteomics we gain insights into the underlying molecular mechanisms. We identify substrates of PfCDPK1, which includes proteins of Inner Membrane Complex and glideosome-actomyosin motor assembly. Interestingly, PfCDPK1 phosphorylates PfPKA regulatory subunit (PfPKA-R) and regulates PfPKA activity in the parasite, which may be relevant for the process of invasion. This study delineates the signaling network of PfCDPK1 and sheds light on mechanisms via which it regulates invasion.

Suggested Citation

  • Sudhir Kumar & Manish Kumar & Roseleen Ekka & Jeffrey D. Dvorin & Aditya S. Paul & Anil K. Madugundu & Tim Gilberger & Harsha Gowda & Manoj T. Duraisingh & T. S. Keshava Prasad & Pushkar Sharma, 2017. "PfCDPK1 mediated signaling in erythrocytic stages of Plasmodium falciparum," Nature Communications, Nature, vol. 8(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00053-1
    DOI: 10.1038/s41467-017-00053-1
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-017-00053-1
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-017-00053-1?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Kieran Tebben & Salif Yirampo & Drissa Coulibaly & Abdoulaye K. Koné & Matthew B. Laurens & Emily M. Stucke & Ahmadou Dembélé & Youssouf Tolo & Karim Traoré & Amadou Niangaly & Andrea A. Berry & Boure, 2024. "Gene expression analyses reveal differences in children’s response to malaria according to their age," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    2. Ravish Rashpa & Natacha Klages & Domitille Schvartz & Carla Pasquarello & Mathieu Brochet, 2023. "The Skp1-Cullin1-FBXO1 complex is a pleiotropic regulator required for the formation of gametes and motile forms in Plasmodium berghei," Nature Communications, Nature, vol. 14(1), pages 1-18, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00053-1. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.