IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v8y2017i1d10.1038_ncomms15940.html
   My bibliography  Save this article

A dynamic and adaptive network of cytosolic interactions governs protein export by the T3SS injectisome

Author

Listed:
  • Andreas Diepold

    (University of Oxford
    Present address: Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Straße 10, 35043 Marburg, Germany)

  • Erdinc Sezgin

    (MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford)

  • Miles Huseyin

    (University of Oxford)

  • Thomas Mortimer

    (University of Oxford
    Present address: The Francis Crick Institute, 1 Midland Road, Kings Cross, London NW1 1AT, UK)

  • Christian Eggeling

    (MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford)

  • Judith P. Armitage

    (University of Oxford)

Abstract

Many bacteria use a type III secretion system (T3SS) to inject effector proteins into host cells. Selection and export of the effectors is controlled by a set of soluble proteins at the cytosolic interface of the membrane spanning type III secretion ‘injectisome’. Combining fluorescence microscopy, biochemical interaction studies and fluorescence correlation spectroscopy, we show that in live Yersinia enterocolitica bacteria these soluble proteins form complexes both at the injectisome and in the cytosol. Binding to the injectisome stabilizes these cytosolic complexes, whereas the free cytosolic complexes, which include the type III secretion ATPase, constitute a highly dynamic and adaptive network. The extracellular calcium concentration, which triggers activation of the T3SS, directly influences the cytosolic complexes, possibly through the essential component SctK/YscK, revealing a potential mechanism involved in the regulation of type III secretion.

Suggested Citation

  • Andreas Diepold & Erdinc Sezgin & Miles Huseyin & Thomas Mortimer & Christian Eggeling & Judith P. Armitage, 2017. "A dynamic and adaptive network of cytosolic interactions governs protein export by the T3SS injectisome," Nature Communications, Nature, vol. 8(1), pages 1-12, August.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15940
    DOI: 10.1038/ncomms15940
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms15940
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms15940?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Dominic Gilzer & Madeleine Schreiner & Hartmut H. Niemann, 2022. "Direct interaction of a chaperone-bound type III secretion substrate with the export gate," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15940. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.